ADAR1 Antibody [E16C6] | Primary Antibodies

Application: Reactivity: Human, Monkey

Lane 1: HepG2, Lane 2: Vero

Usage Information

Dilution
WB1:1000

Application
WB

Reactivity
Human, Monkey

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
110 kDa, 150 kDa

Datasheet & SDS

Biological Description

Specificity
ADAR1 Antibody [E16C6] detects endogenous levels of total ADAR1 protein.

Clone
E16C6

Synonym(s)
Double-strand adenosine deaminase; DRADA; 136 kDa double-stranded RNA-binding protein (p136); Interferon-inducible protein 4 (IFI-4); K88DSRBP; ADAR; ADAR1; DSRAD; G1P1; IFI4

Background
ADAR1 (adenosine deaminase acting on RNA 1) is a double‑stranded RNA‑specific adenosine deaminase that belongs to the ADAR family and catalyzes the conversion of adenosine to inosine (A‑to‑I editing) in structured RNA regions, a process that is interpreted as an A‑to‑G change by the splicing and translational machinery and thereby diversifies transcript and protein isoforms without altering the genomic sequence. It exists as two major isoforms, ADAR1L (p150) and ADAR1S (p110), generated from alternative promoters and translation start sites, with ADAR1S localized constitutively in the nucleus and ADAR1L being inducible by interferon and present in both the nucleus and the cytoplasm, where it can engage overlapping but non‑identical substrate pools. ADAR1‑mediated editing modulates sequence recognition by RNA‑binding proteins and non‑coding RNAs, including microRNAs, and thereby influences alternative splicing, mRNA stability, translational efficiency, and the targeting of regulatory RNAs. ADAR1 functions as a key suppressor of aberrant interferon responses by editing endogenous double‑stranded RNAs so that they are not recognized as foreign by cytoplasmic RNA sensors such as MDA5 and RIG‑I; loss or inhibition of ADAR1 leads to accumulation of unedited or hyperedited substrates that trigger type‑I and type‑II interferon signaling and inflammation. ADAR1 is also essential for their maintenance in the fetal liver and adult bone marrow, and ADAR1 deficiency in these cells causes global upregulation of interferon‑stimulated genes and rapid apoptosis.

Background

ADAR1 (adenosine deaminase acting on RNA 1) is a double‑stranded RNA‑specific adenosine deaminase that belongs to the ADAR family and catalyzes the conversion of adenosine to inosine (A‑to‑I editing) in structured RNA regions, a process that is interpreted as an A‑to‑G change by the splicing and translational machinery and thereby diversifies transcript and protein isoforms without altering the genomic sequence. It exists as two major isoforms, ADAR1L (p150) and ADAR1S (p110), generated from alternative promoters and translation start sites, with ADAR1S localized constitutively in the nucleus and ADAR1L being inducible by interferon and present in both the nucleus and the cytoplasm, where it can engage overlapping but non‑identical substrate pools. ADAR1‑mediated editing modulates sequence recognition by RNA‑binding proteins and non‑coding RNAs, including microRNAs, and thereby influences alternative splicing, mRNA stability, translational efficiency, and the targeting of regulatory RNAs. ADAR1 functions as a key suppressor of aberrant interferon responses by editing endogenous double‑stranded RNAs so that they are not recognized as foreign by cytoplasmic RNA sensors such as MDA5 and RIG‑I; loss or inhibition of ADAR1 leads to accumulation of unedited or hyperedited substrates that trigger type‑I and type‑II interferon signaling and inflammation. ADAR1 is also essential for their maintenance in the fetal liver and adult bone marrow, and ADAR1 deficiency in these cells causes global upregulation of interferon‑stimulated genes and rapid apoptosis.

References
https://pubmed.ncbi.nlm.nih.gov/19060901/ https://pubmed.ncbi.nlm.nih.gov/25456137/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%