Abi1 Antibody [D2H9] | Primary Antibodies

Application: Reactivity: Human, Mouse, Rat

Lane 1: NIH/3T3, Lane 2: Neuro-2a, Lane 3: J774A.1, Lane 4: PC12

Usage Information

Dilution
WB1:1000 IP1:100

Application
WB, IP

Reactivity
Human, Mouse, Rat

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
55-65 kDa

Datasheet & SDS

Biological Description

Specificity
Abi1 Antibody [D2H9] detects endogenous levels of total Abi1 protein.

Clone
D2H9

Synonym(s)
Abl interactor 1; Abelson interactor 1; Abi-1; Spectrin-binding protein e3B1; ABI1

Background
Abelson interactor 1 (Abi1, ABI1) is an adaptor protein of the Abi family that functions downstream of receptor‑ or growth‑factor‑activated tyrosine kinases, including c‑Abl, and integrates inputs into actin‑dependent cytoskeletal remodeling and growth‑factor signaling. Abi1 contains multiple modular domains that support its scaffolding function: a WAVE‑binding region that recruits WAVE complex subunits such as WAVE2, SH3 domains that engage proline‑rich motifs on c‑Abl, and a PXXP sequence together with a PEST‑like region that can be phosphorylated to create docking sites for SH2‑containing signaling proteins. Abi1 acts as a core element of the WAVE regulatory complex, promoting Rac‑driven actin polymerization and lamellipodial protrusion, and it also participates in alternative complexes with SOS1 and EPS8 that link Ras activation to Rac and actin remodeling, thereby coordinating directional migration and invadopodia formation. In the context of growth‑factor and kinase signaling, c‑Abl phosphorylates Abi1 at Y213, generating a phosphotyrosine motif that independently recruits the SH2 domains of both c‑Abl and the p85 regulatory subunit of PI3K, thus coupling Abi‑mediated cytoskeletal dynamics to PI3K‑Akt activation and other PI3K‑dependent outputs. Phosphorylation of Abi1 at Y435 enhances tumor‑cell adhesion, extracellular‑matrix degradation, and invasive behavior, and this modification is tightly regulated by c‑Abl, making Abi1 a mechanistic node that links Abl kinase activity to actin‑driven invasion and matrix remodeling in colorectal and other carcinomas.

Background

Abelson interactor 1 (Abi1, ABI1) is an adaptor protein of the Abi family that functions downstream of receptor‑ or growth‑factor‑activated tyrosine kinases, including c‑Abl, and integrates inputs into actin‑dependent cytoskeletal remodeling and growth‑factor signaling. Abi1 contains multiple modular domains that support its scaffolding function: a WAVE‑binding region that recruits WAVE complex subunits such as WAVE2, SH3 domains that engage proline‑rich motifs on c‑Abl, and a PXXP sequence together with a PEST‑like region that can be phosphorylated to create docking sites for SH2‑containing signaling proteins. Abi1 acts as a core element of the WAVE regulatory complex, promoting Rac‑driven actin polymerization and lamellipodial protrusion, and it also participates in alternative complexes with SOS1 and EPS8 that link Ras activation to Rac and actin remodeling, thereby coordinating directional migration and invadopodia formation. In the context of growth‑factor and kinase signaling, c‑Abl phosphorylates Abi1 at Y213, generating a phosphotyrosine motif that independently recruits the SH2 domains of both c‑Abl and the p85 regulatory subunit of PI3K, thus coupling Abi‑mediated cytoskeletal dynamics to PI3K‑Akt activation and other PI3K‑dependent outputs. Phosphorylation of Abi1 at Y435 enhances tumor‑cell adhesion, extracellular‑matrix degradation, and invasive behavior, and this modification is tightly regulated by c‑Abl, making Abi1 a mechanistic node that links Abl kinase activity to actin‑driven invasion and matrix remodeling in colorectal and other carcinomas.

References
https://pubmed.ncbi.nlm.nih.gov/24913355/ https://pubmed.ncbi.nlm.nih.gov/23558677/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%