Thymopentin

Catalog No.S3668 Batch:S366801

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Technical Data

Formula

C30H49N9O9
Molecular Weight 679.77 CAS No. 69558-55-0
Solubility (25°C)* In vitro DMSO 100 mg/mL (147.1 mM)
Water 100 mg/mL (147.1 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Thymopentin (TP5) has immuno-regulatory activities. The immuno-regulatory actions of thymopentin on peripheral T cells are mediated by intracellular cyclic GMP elevations in contrast to the intracellular cyclic AMP elevations induced in precursor T cells that trigger their further differentiation to T cells.
In vitro TP5 displays concentration-dependent inhibitory effects on the proliferation and colony formation of HL-60 cells. the decrease or even disappearance of AgNORs from nucleoli is observed in HL-60 cells after the treatment with TP5. The suppression induced by TP5 is accompanied by an accumulation of cell cycle in the G0/G1 phase. Moreover, TP5 significantly increases the NBT-reduction activity of HL-60 cells. TP5 has induced differentiation along the granulocytes lineage in HL-60 cells[1]. TP5 increases the expression of CD4 and CD8 markers on the surface of human thymocytes.
In vivo TP5 is able to markedly stimulate the activity of immune system cells in healthy animals. It activates the production of a number of cytokines (IL1α, IL2, IL6, IL10, IFNγ), NO, Hsp70 and Hsp90 in mature cells of the peripheral immune system of mice (peritoneal macrophages and spleen lymphocytes)[2].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    HL-60 cells

  • Concentrations

    100–800 μmol/L

  • Incubation Time

    120 h

  • Method

    HL-60 cells are plated at a density of 1 × 105 cells/mL, and cultured for 120h in a medium containing TP5 at various concentrations (100-800 μmol/L). To quantify the number of cells after treatment, cells are counted at different time points of incubation using a hemocytometer under a light microscope. Cell viability is determined by the Trypan blue exclusion assay. Viability is calculated as the ratio of living cells to the total cell population.
Animal Study:[2]
  • Animal Models

    NMRI mice

  • Dosages

    15 µg/100 g

  • Administration

    i.p.

Selleck's Thymopentin has been cited by 1 publication

Novel Natural Inhibitors Targeting Enhancer of Zeste Homolog 2: A Comprehensive Structural Biology Research [ Front Oncol, 2021, 11:741403] PubMed: 34737956

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.