Colchicine

Catalog No.S2284 Batch:S228404

Technical Data

Formula	C₂₂H₂₅NO₆
Molecular Weight	399.44	CAS No.	64-86-8
Solubility (25°C)*	In vitro	DMSO	79 mg/mL (197.77 mM)
		Water	79 mg/mL (197.77 mM)
		Ethanol	79 mg/mL (197.77 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.
In vitro	Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.^[1]
In vivo	Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HeLa cells Concentrations 5 μM Incubation Time 2 h Method HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed.

Cell Assay:^{^[1]}

Cell lines
HeLa cells
Concentrations
5 μM
Incubation Time
2 h
Method
HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed.

References

[1] Niu L, et al. J Biol Chem. 2019 Nov 29;294(48):18099-18108.

Selleck's Colchicine has been cited by 21 publications

Novel Brain-Penetrant, Small-Molecule Tubulin Destabilizers for the Treatment of Glioblastoma [ Biomedicines, 2024, 12(2)406]	PubMed: 38398008
Restricting epigenetic activity promotes the reprogramming of transformed cells to pluripotency in a line-specific manner [ Cell Death Discov, 2023, 9(1):245]	PubMed: 37452056
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
Intravital Imaging Reveals the Ameliorating Effect of Colchicine in a Photothrombotic Stroke Model via Inhibition of Neutrophil Recruitment [ Transl Stroke Res, 2022, 10.1007/s12975-022-01022-7]	PubMed: 35441983
Colchicine Ameliorates Dilated Cardiomyopathy Via SIRT2-Mediated Suppression of NLRP3 Inflammasome Activation [ J Am Heart Assoc, 2022, 11(13):e025266]	PubMed: 35766262
IMB5476, a novel microtubule inhibitor, induces mitotic catastrophe and overcomes multidrug resistance in tumors [ Eur J Pharmacol, 2022, 919:174802]	PubMed: 35143830
Colchicine alleviates inflammation and improves diastolic dysfunction in heart failure rats with preserved ejection fraction [ Eur J Pharmacol, 2022, 929:175126]	PubMed: 35779623
Alpha-synuclein/MPP+ mediated activation of NLRP3 inflammasome through microtubule-driven mitochondrial perinuclear transport [ Biochem Biophys Res Commun, 2022, 594:161-167]	PubMed: 35085893
Reciprocal interaction between SIRT6 and APC/C regulates genomic stability [ Sci Rep, 2021, 11(1):14253]	PubMed: 34244565

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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