Azelaic acid

Catalog No.S4550 Batch:S455001

Technical Data

Formula	C₉H₁₆O₄
Molecular Weight	188.22	CAS No.	123-99-9
Solubility (25°C)*	In vitro	DMSO	37 mg/mL (196.57 mM)
		Water	37 mg/mL (196.57 mM)
		Ethanol	37 mg/mL (196.57 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Azelaic acid (Nonanedioic acid, Finacea, Azelex, Anchoic acid) is an organic compound produced by the ozonolysis of oleic acid and possesses antibacterial, keratolytic, comedolytic, and anti-oxidant activity.
In vitro	AZ(Azelaic acid) combined with taurine (Tau) show more inhibitory effects in melanocytes than the treatment of AZ alone. AZ combined with Tau inhibits the melanin production and tyrosinase activity of B16F10 melanoma cells without significant cytotoxicity. Also inhibitory effects after treatment with these combined chemical are stronger than AZ alone on melanogenesis^[2].
In vivo	Azelaic acid exhibits an anti-atherogenic effect. Its athero-protective effect is not related to changes in plasma lipid content. Azelaic acid supplementation decreases the level of CD68 macrophage marker by 34% (p<0.05)^[1].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines The mouse melanoma cell line B16F10 Concentrations 20 mM Incubation Time 24 h Method B16F10 melanoma cells are seeded at a density of 2.5×10⁵ cells/60 mm culture dish. The cells are treated with Azelaic acid combined with Tau for 24 hr. The cells pellets are dissolved in 1 N NaOH at 60°C for 1 hr. The relative melanin content is determined by measuring the absorbance at 475 nm in ELISA reader.
Animal Study:^{^[1]}	Animal Models Low density lipoprotein receptor knockout (LDLr−/−) mice Dosages 0.50% Administration oral administration

Cell Assay:^{^[2]}

Cell lines
The mouse melanoma cell line B16F10
Concentrations
20 mM
Incubation Time
24 h
Method
B16F10 melanoma cells are seeded at a density of 2.5×10⁵ cells/60 mm culture dish. The cells are treated with Azelaic acid combined with Tau for 24 hr. The cells pellets are dissolved in 1 N NaOH at 60°C for 1 hr. The relative melanin content is determined by measuring the absorbance at 475 nm in ELISA reader.

Animal Study:^{^[1]}

Animal Models
Low density lipoprotein receptor knockout (LDLr−/−) mice
Dosages
0.50%
Administration
oral administration

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.