WST-8

Catalog No.S7764 Batch:S776414

Technical Data

Formula	C₂₀H₁₃N₆NaO₁₁S₂
Molecular Weight	600.47	CAS No.	193149-74-5
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (166.53 mM)
		Water	3 mg/mL (4.99 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity that produces corresponding formazan dye that absorbs at 460 nm. WST-8 is typically used as a cell viability indicator in cell proliferation assays.
In vitro	WST-8 is reduced by NADH in good yields at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nm. The formazan is soluble to water at concentrations higher than 0.1 M. WST-8 is useful as a sensitive chromogenic indicator for NADH. It is also applicable to cell proliferation assays as a cell viability indicator.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Hela cells Concentrations 0.5 mM Incubation Time 2 h Method A hundred μl of HeLa cell culture in the logarithmic phase is inoculated onto a 96-well microtiter plate, and the plate pre-incubated for 24 h in a CO₂ incubator at 37℃. A 10 μl of the working solution containing WST-8 and lmethoxy PMS (0.5 mM and 20 μM, respectively, as the final concentration) is added to each well; it is incubated for an additional 2 h. The absorbance of each well is measured at 450 nm with a reference wavelength at 650 nm with an M-UVmax microplate reader.

Cell Assay:^{^[1]}

Cell lines
Hela cells
Concentrations
0.5 mM
Incubation Time
2 h
Method
A hundred μl of HeLa cell culture in the logarithmic phase is inoculated onto a 96-well microtiter plate, and the plate pre-incubated for 24 h in a CO₂ incubator at 37℃. A 10 μl of the working solution containing WST-8 and lmethoxy PMS (0.5 mM and 20 μM, respectively, as the final concentration) is added to each well; it is incubated for an additional 2 h. The absorbance of each well is measured at 450 nm with a reference wavelength at 650 nm with an M-UVmax microplate reader.

References

[1] Ishiyama M, et al. Talanta. 1997 Jul;44(7):1299-305.

Selleck's WST-8 has been cited by 4 publications

Anti-PTK7 Monoclonal Antibodies Exhibit Anti-Tumor Activity at the Cellular Level and in Mouse Xenograft Models of Esophageal Squamous Cell Carcinoma [ Int J Mol Sci, 2022, 23-2012195]	PubMed: 36293051
Anti-PTK7 Monoclonal Antibodies Exhibit Anti-Tumor Activity at the Cellular Level and in Mouse Xenograft Models of Esophageal Squamous Cell Carcinoma [ Int J Mol Sci, 2022, 23-2012195]	PubMed: 36293051
Curcumin Decreases Viability and Inhibits Proliferation of Imatinib-Sensitive and Imatinib-Resistant Chronic Myeloid Leukemia Cell Lines [ Metabolites, 2022, 13(1)58]	PubMed: 36676983
Lactate enhances Arc/arg3.1 expression through hydroxycarboxylic acid receptor 1-β-arrestin2 pathway in astrocytes. [ Neuropharmacology, 2020, 12;171:108084]	PubMed: 32294462

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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