UQCRC2 Antibody [C21D5] Datasheet

Biological Description

Specificity	UQCRC2 Antibody [C21D5] detects endogenous levels of total UQCRC2 protein.
Background	UQCRC2 (ubiquinol‑cytochrome c reductase core protein 2) is a nuclear‑encoded structural subunit of the mitochondrial cytochrome b‑c1 complex (Complex III) that is embedded in the inner mitochondrial membrane and contributes to the assembly, stability, and electron‑transport activity of the ubiquinol‑cytochrome c oxidoreductase complex. Within the electron transport chain, UQCRC2‑containing Complex III transfers electrons from ubiquinol to cytochrome c, coupling this redox reaction to proton translocation across the inner membrane and supporting the electrochemical gradient that drives ATP synthesis and mitochondrial integrity. UQCRC2 expression is transcriptionally regulated by AMP‑activated protein kinase (AMPK) through activation of the transcription factor NFE2L2/NRF2, and this AMPK‑NRF2–UQCRC2 axis enhances UQCRC2 levels and promotes mitophagy, the selective clearance of damaged mitochondria, thereby protecting hepatocytes against alcohol‑induced liver injury. UQCRC2 is downregulated in tumor tissue compared with adjacent non‑carcinoma tissue, and its expression correlates inversely with lymph node metastasis, relapse, and tumor grade; UQCRC2 overexpression suppresses gastric cancer cell proliferation, migration, and invasion in vitro and in vivo, whereas reduction of UQCRC2 by upstream miRNAs such as miR‑370 enhances tumor aggressiveness.

Specificity

UQCRC2 Antibody [C21D5] detects endogenous levels of total UQCRC2 protein.

Background

UQCRC2 (ubiquinol‑cytochrome c reductase core protein 2) is a nuclear‑encoded structural subunit of the mitochondrial cytochrome b‑c1 complex (Complex III) that is embedded in the inner mitochondrial membrane and contributes to the assembly, stability, and electron‑transport activity of the ubiquinol‑cytochrome c oxidoreductase complex. Within the electron transport chain, UQCRC2‑containing Complex III transfers electrons from ubiquinol to cytochrome c, coupling this redox reaction to proton translocation across the inner membrane and supporting the electrochemical gradient that drives ATP synthesis and mitochondrial integrity. UQCRC2 expression is transcriptionally regulated by AMP‑activated protein kinase (AMPK) through activation of the transcription factor NFE2L2/NRF2, and this AMPK‑NRF2–UQCRC2 axis enhances UQCRC2 levels and promotes mitophagy, the selective clearance of damaged mitochondria, thereby protecting hepatocytes against alcohol‑induced liver injury. UQCRC2 is downregulated in tumor tissue compared with adjacent non‑carcinoma tissue, and its expression correlates inversely with lymph node metastasis, relapse, and tumor grade; UQCRC2 overexpression suppresses gastric cancer cell proliferation, migration, and invasion in vitro and in vivo, whereas reduction of UQCRC2 by upstream miRNAs such as miR‑370 enhances tumor aggressiveness.

Usage Information

Application

WB, IP

Dilution

WB	IP
1:1000	1:50

Reactivity

Human, Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

48 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/33719895/
https://pubmed.ncbi.nlm.nih.gov/32742452/

Application Data

WB

Validated by Selleck

Lane 1: HepG2, Lane 2: Huh7, Lane 3: A172, Lane 4: 293T