UBCS039

Catalog No.S8909 Batch:S890901

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Technical Data

Formula

C16H13N3
Molecular Weight 247.29 CAS No. 358721-70-7
Solubility (25°C)* In vitro DMSO 49 mg/mL (198.14 mM)
Ethanol 16 mg/mL (64.7 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.45mg/ml Taking the 1 mL working solution as an example, add 50 μL of 49 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
1.21mg/ml Taking the 1 mL working solution as an example, add 50 μL of 24.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description UBCS039 is the first synthetic SIRT6 activator with EC50 of 38 μM,induces a time-dependent activation of autophagy in several human tumor cell lines.
Targets
SIRT6 [1]
(Cell-free assay)
38 μM(EC50)
In vitro

UBCS039 induces deacetylation of SIRT6-targeted histone H3 sites in human cancer cells.UBCS039 leads to autophagosome accumulation and induces autophagic flux in human cancer cells.UBCS039 also induces autophagy-associated cell death.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HeLa cells

  • Concentrations

    100 µM

  • Incubation Time

    24 h

  • Method

    HeLa cells are treated with 100 µM UBCS039 for 24 h, harvested, washed in PBS 1× and stained for 30 min at 37 °C with dihydroethidium 50 µM dissolved in DMEM without fetal bovine serum.

Selleck's UBCS039 has been cited by 4 publications

SIRT6 mediates MRTF-A deacetylation in vascular endothelial cells to antagonize oxLDL-induced ICAM-1 transcription [ Cell Death Discov, 2022, 8(1):96] PubMed: 35246513
Downregulation of Sirt6 by CD38 promotes cell senescence and aging [ Aging (Albany NY), 2022, 14(23):9730-9757] PubMed: 36490326
Downregulation of Sirt6 by CD38 promotes cell senescence and aging [ Aging (Albany NY), 2022, 14(23):9730-9757] PubMed: 36490326
Virtual Screening in the Identification of Sirtuins' Activity Modulators [ Molecules, 2022, 27-175641] PubMed: 36080416

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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