TLR4-IN-C34

Catalog No.S0822 Batch:S082201

Technical Data

Formula	C₁₇H₂₇NO₉
Molecular Weight	389.40	CAS No.	40592-88-9
Solubility (25°C)*	In vitro	DMSO	78 mg/mL (200.3 mM)
		Water	78 mg/mL (200.3 mM)
		Ethanol	78 mg/mL (200.3 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

TLR4-IN-C34 (TLR4-C34, C34, TLR4-C34-IN) is a potent and selective antagonist of Toll-like receptor 4 (TLR4). TLR4-IN-C34 reduces systemic inflammation in mouse models of endotoxemia and necrotizing enterocolitis.

Targets

TLR4 ^[1]

In vitro

TLR4-IN-C34 inhibits TLR4 in vitro in macrophages and enterocytes via direct binding.^[1]

In vivo

TLR4-IN-C34 can attenuate NEC severity and demonstrates a marked preservation of the intestinal mucosa.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines IEC-6 cells, RAW 264.7 cells Concentrations 10 μM Incubation Time 30 min Method IEC-6 cells and RAW 264.7 cells are treated withTLR4-IN-C34 at a concentration of 10 μM 30 min prior to treatment with LPS, and the extent of LPS signaling is determined by the degree of TNFα expression by qRT-PCR. In parallel, RAW 264.7 cells are transduced with an adenovirus expressing the NFκB-luciferase reporter gene, and then treated with LPS at 10 ng/ml after pre-treatment with TLR4-IN-C34 at 100 µM. The NFκB-luciferase activity is measured using the Luciferase Activity System.

Cell Assay:^{^[1]}

Cell lines
IEC-6 cells, RAW 264.7 cells
Concentrations
10 μM
Incubation Time
30 min
Method
IEC-6 cells and RAW 264.7 cells are treated withTLR4-IN-C34 at a concentration of 10 μM 30 min prior to treatment with LPS, and the extent of LPS signaling is determined by the degree of TNFα expression by qRT-PCR. In parallel, RAW 264.7 cells are transduced with an adenovirus expressing the NFκB-luciferase reporter gene, and then treated with LPS at 10 ng/ml after pre-treatment with TLR4-IN-C34 at 100 µM. The NFκB-luciferase activity is measured using the Luciferase Activity System.

References

Selleck's TLR4-IN-C34 has been cited by 2 publications

Nucleus pulposus cells regulate macrophages in degenerated intervertebral discs via the integrated stress response-mediated CCL2/7-CCR2 signaling pathway [ Exp Mol Med, 2024, 56(2):408-421.]	PubMed: 38316963
Oxidative stress mediated by the TLR4/NOX2 signalling axis is involved in polystyrene microplastic-induced uterine fibrosis in mice [ Sci Total Environ, 2022, 838(Pt 2):155825]	PubMed: 35597360

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SHIPPING AND STORAGE
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