Ochromycinone (STA-21)

Catalog No.S7951 Batch:S795102

Technical Data

Formula	C₁₉H₁₄O₄
Molecular Weight	306.31	CAS No.	111540-00-2
Solubility (25°C)*	In vitro	DMSO	21 mg/mL (68.55 mM)
		Ethanol	2 mg/mL (6.52 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Ochromycinone (STA-21) is a selective STAT3 inhibitor.

Targets

STAT3 ^[1]

In vitro

In cells, STA-21 inhibits Stat3 DNA binding activity, Stat3 dimerization, and Stat3-dependent luciferase activity. STA-21 remarkably inhibits the growth and the survival of the breast carcinoma cells MDA-MB-231, MDA-MB-435s, and MDA-MB-468 that express persistently activated Stat3. ^[1] In RH30 and RD2 cells, STA-21 also inhibits cell viability and growth and induced apoptosis through caspases 3, 8 and 9 pathways. ^[2]

In vivo

In IL-1Ra–KO mice, STA-21 (0.5 mg/kg, i.p.) reduces the arthritis score and the incidence of arthritis by decreasing the proportion of Th17 cells and increasing the proportion of Treg cells expressing FoxP3. ^[3]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, MCF7, and Caov-3 cells Concentrations 20 or 30 μM Incubation Time 48 h Method Human breast cancer cell lines MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, and MCF7, human ovarian carcinoma cell line Caov-3, and human skin fibroblasts were cultured in DMEM containing 10% FBS and appropriate antibiotics in a 5% CO₂ incubator at 37°C. For testing different compounds, the cells were exposed to the compounds for 48 h at a final concentration of 20 or 30 μM, respectively. Then, the cells were harvested and subjected to flow-cytometry analysis.
Animal Study:^{^[3]}	Animal Models IL-1Ra–KO mice Dosages 0.5 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, MCF7, and Caov-3 cells
Concentrations
20 or 30 μM
Incubation Time
48 h
Method
Human breast cancer cell lines MDA-MB-231, MDA-MB-435s, MDA-MB-453, MDA-MB-468, and MCF7, human ovarian carcinoma cell line Caov-3, and human skin fibroblasts were cultured in DMEM containing 10% FBS and appropriate antibiotics in a 5% CO₂ incubator at 37°C. For testing different compounds, the cells were exposed to the compounds for 48 h at a final concentration of 20 or 30 μM, respectively. Then, the cells were harvested and subjected to flow-cytometry analysis.

Animal Study:^{^[3]}

Animal Models
IL-1Ra–KO mice
Dosages
0.5 mg/kg
Administration
i.p.

References

Selleck's Ochromycinone (STA-21) has been cited by 8 publications

Cationic amphiphilic antihistamines inhibit STAT3 via Ca2+-dependent lysosomal H+ efflux [ Cell Rep, 2023, 42(2):112137]	PubMed: 36807142
Microglial depletion impairs glial scar formation and aggravates inflammation partly by inhibiting STAT3 phosphorylation in astrocytes after spinal cord injury [ Neural Regen Res, 2023, 18(6):1325-1331]	PubMed: 36453419
TTI-101: A competitive inhibitor of STAT3 that spares oxidative phosphorylation and reverses mechanical allodynia in mouse models of neuropathic pain [ Biochem Pharmacol, 2021, 192:114688]	PubMed: 34274354
The nuclear gene rpl18 regulates erythroid maturation via JAK2-STAT3 signaling in zebrafish model of Diamond-Blackfan anemia. [ Cell Death Dis, 2020, 19;11(2):135]	PubMed: 32075953
Interleukin-6 Derived From Cancer-Associated Fibroblasts Attenuates the p53 Response to Doxorubicin in Prostate Cancer Cells [ Cell Death Discov, 2020, 2;6:42]	PubMed: 32528731
Combination of ERK2 and STAT3 Inhibitors Promotes Anticancer Effects on Acute Lymphoblastic Leukemia Cells [ Cancer Genomics Proteomics, 2020, 17(5):517-527]	PubMed: 32859630
JAK/STAT3 and NF-κB Signaling Pathways Regulate Cancer Stem-Cell Properties in Anaplastic Thyroid Cancer Cells. [ Thyroid, 2019, 29(5):674-682]	PubMed: 30784360
STAT3 regulates the onset of oxidant-induced senescence in lung fibroblasts [Waters DW Am J Respir Cell Mol Biol, 2019, 10.1165/rcmb.2018-0328OC]	PubMed: 30608861

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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