SN-011

Catalog No.E1066 Batch:E106603

Technical Data

Formula	C₂₅H₁₉FN₂O₄S
Molecular Weight	462.49	CAS No.	2249435-90-1
Solubility (25°C)*	In vitro	DMSO	93 mg/mL (201.08 mM)
		Ethanol	6 mg/mL (12.97 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

SN-011 is a STING-specific antagonist with IC50 of 76 nM.

Targets

STING ^[1]

76 nM

In vitro

SN-011 substantially suppresses 2′3′-cGAMP-induced STING oligomerization and phosphorylation assessed by immunoblot in HFFs. SN-011 specifically inhibits STING activation and STING-dependent signaling.^[1]

In vivo

Serum antinuclear antibody is markedly reduced by SN-011 treatment. SN-011 also significantly reduces the number of activated CD69⁺ CD8 T cells and memory CD44^high CD62L^low CD4 and CD8 T cells to near normal levels in the spleens of treated Trex1^−/− mice. SN-011 strongly reduces inflammation and protects Trex1^−/− mice from death.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HFFs Concentrations 1 μM - 10 μM Incubation Time 24 h Method HFFs are harvested and lysed in lysis buffer with brief sonication. Cell lysate is collected and incubated with biotin (5 μM). For purified recombinant STING-CTD protein, 0.05 mg protein in 400 μL buffer (25 mM Tris-HCl, pH 7.5, 150 mM NaCl) was incubated with biotin (5 μM) for 1 h at 4 °C. Then the protein of interest was pull-down by streptavidinconjugated agarose and detected by immunoblot analysis.
Animal Study:^{^[1]}	Animal Models Trex1^−/− mice Dosages 5 mg/kg, 10 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
HFFs
Concentrations
1 μM - 10 μM
Incubation Time
24 h
Method
HFFs are harvested and lysed in lysis buffer with brief sonication. Cell lysate is collected and incubated with biotin (5 μM). For purified recombinant STING-CTD protein, 0.05 mg protein in 400 μL buffer (25 mM Tris-HCl, pH 7.5, 150 mM NaCl) was incubated with biotin (5 μM) for 1 h at 4 °C. Then the protein of interest was pull-down by streptavidinconjugated agarose and detected by immunoblot analysis.

Animal Study:^{^[1]}

Animal Models
Trex1^−/− mice
Dosages
5 mg/kg, 10 mg/kg
Administration
i.p.

References

[1] Ze Hong, et al. Proc Natl Acad Sci U S A . 2021 Jun 15;118(24):e2105465118.

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SHIPPING AND STORAGE
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