SN-011

Catalog No.E1066 Batch:E106602

Technical Data

Formula

C₂₅H₁₉FN₂O₄S

Molecular Weight

462.49

CAS No.

2249435-90-1

Solubility (25°C)*

In vitro

DMSO

93 mg/mL (201.08 mM)

Ethanol

5 mg/mL (10.81 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% corn oil	0.45mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 9 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.65mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 93 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

SN-011 is a STING-specific antagonist with IC50 of 76 nM.

Targets

STING ^[1]

76 nM

In vitro

SN-011 substantially suppresses 2′3′-cGAMP-induced STING oligomerization and phosphorylation assessed by immunoblot in HFFs. SN-011 specifically inhibits STING activation and STING-dependent signaling.^[1]

In vivo

Serum antinuclear antibody is markedly reduced by SN-011 treatment. SN-011 also significantly reduces the number of activated CD69⁺ CD8 T cells and memory CD44^high CD62L^low CD4 and CD8 T cells to near normal levels in the spleens of treated Trex1^−/− mice. SN-011 strongly reduces inflammation and protects Trex1^−/− mice from death.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HFFs Concentrations 1 μM - 10 μM Incubation Time 24 h Method HFFs are harvested and lysed in lysis buffer with brief sonication. Cell lysate is collected and incubated with biotin (5 μM). For purified recombinant STING-CTD protein, 0.05 mg protein in 400 μL buffer (25 mM Tris-HCl, pH 7.5, 150 mM NaCl) was incubated with biotin (5 μM) for 1 h at 4 °C. Then the protein of interest was pull-down by streptavidinconjugated agarose and detected by immunoblot analysis.
Animal Study:^{^[1]}	Animal Models Trex1^−/− mice Dosages 5 mg/kg, 10 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
HFFs
Concentrations
1 μM - 10 μM
Incubation Time
24 h
Method
HFFs are harvested and lysed in lysis buffer with brief sonication. Cell lysate is collected and incubated with biotin (5 μM). For purified recombinant STING-CTD protein, 0.05 mg protein in 400 μL buffer (25 mM Tris-HCl, pH 7.5, 150 mM NaCl) was incubated with biotin (5 μM) for 1 h at 4 °C. Then the protein of interest was pull-down by streptavidinconjugated agarose and detected by immunoblot analysis.

Animal Study:^{^[1]}

Animal Models
Trex1^−/− mice
Dosages
5 mg/kg, 10 mg/kg
Administration
i.p.

References

[1] Ze Hong, et al. Proc Natl Acad Sci U S A . 2021 Jun 15;118(24):e2105465118.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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