Silver Sulfadiazine

Catalog No.S4822 Batch:S482201

Technical Data

Formula	C₁₀H₉AgN₄O₂S
Molecular Weight	357.14	CAS No.	22199-08-2
Solubility (25°C)*	In vitro	4-Methylpyridine	3 mg/mL warmed with 50ºC water bath (8.4 mM)
		DMSO	0.1 mg/mL (0.28 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Silver sulfadiazine is a bactericidal for many gram- negative and gram-positive bacteria as well as being effective against yeast.
In vitro	The inhibitory effect of Ag-SD on keratinocyte and fibroblast growth induced the retardation of wound closure rate^[1].
In vivo	Silver sulfadiazine is considered the gold standard for burn treatment^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HaCaT cells Concentrations 0.5%-1% Incubation Time 1 week Method Human HaCaT keratinocytes were incubated in 2-ml Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum. The cultures were maintained up to 80% confluence in 35-mm petridish before treating with Ag-SD and EGF. Samples of collagen sponge disk (0.2 cm²) were prepared by soaking in different concentrations of Ag-SD and/or EGF solutions and freeze-dried. Before usage, the sponges were sterilized by g-irradiation. The sterile sponges of 30-mm diameter were placed in 6-well tissue culture plate, seeded with 0.8×10⁵ HaCaT cells per sponge and cultured in 5% CO2 and 95% humidity for 1 week. Cell viability was assessed by the MTT method.

Cell Assay:^{^[1]}

Cell lines
HaCaT cells
Concentrations
0.5%-1%
Incubation Time
1 week
Method
Human HaCaT keratinocytes were incubated in 2-ml Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum. The cultures were maintained up to 80% confluence in 35-mm petridish before treating with Ag-SD and EGF. Samples of collagen sponge disk (0.2 cm²) were prepared by soaking in different concentrations of Ag-SD and/or EGF solutions and freeze-dried. Before usage, the sponges were sterilized by g-irradiation. The sterile sponges of 30-mm diameter were placed in 6-well tissue culture plate, seeded with 0.8×10⁵ HaCaT cells per sponge and cultured in 5% CO2 and 95% humidity for 1 week. Cell viability was assessed by the MTT method.

References

[1] Cho Lee AR, et al. Biomaterials. 2005, 26(22):4670-6.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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