Rimiducid (AP1903)

Catalog No.S9726 Batch:S972601

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Technical Data

Formula

C78H98N4O20
Molecular Weight 1411.63 CAS No. 195514-63-7
Solubility (25°C)* In vitro DMSO 100 mg/mL (70.84 mM)
Ethanol 100 mg/mL (70.84 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Rimiducid (AP1903) is a chemical protein dimerizer and binds tightly to the target FKBP fusion protein while binding minimally to the abundant natural FKBP. Rimiducid elicites potent and dose-dependent apoptosis of cells in culture expressing dimerizer-dependent Fas constructs, with an EC50 of ≈0.1 nM.
Targets
FKBP fusion protein [1]
In vitro

Rimiducid (AP1903) induces effective and dose-dependent apoptosis in the culture expressing dimer-dependent Fas constructors, indicating that Rimiducid causes the activation of Fas signaling without interference from endogenous FKBP.<sup><a class="sref" href="#s_ref">[2]</a></sup>
In vivo

Rimiducid (AP1903) elicits a dose-dependent decrease in serum hGH levels, with a half-maximal effective dose of 0.4±0.1 mg/kg, which means AP1903 is functional of activating of Fas signaling in vivo.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Assay:

<sup><a class="sref" href="#s_ref">[2]</a></sup>
  • Cell lines

    HT1080 cells

  • Concentrations

    0.01-1000 nM

  • Incubation Time

    Overnight

  • Method

    HT1080 cells stably transduced with retrovirus pSR a-myr-2FKBP-Fas-E (open circles) or pSR a-myr-2 (F36V-FKBP)- Fas-E (closed circles) were treated overnight with AP1903, and viability then was measured by Alamar Blue assay.
Animal Study:

<sup><a class="sref" href="#s_ref">[2]</a></sup>
  • Animal Models

    Nude mice implanted with hGH-secreting HTFasGH-3 cells

  • Dosages

    2 ml/kg

  • Administration

    i.v.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.