Pentylenetetrazol

Catalog No.S4587 Batch:S458703

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Technical Data

Formula

C6H10N4
Molecular Weight 138.17 CAS No. 54-95-5
Solubility (25°C)* In vitro DMSO 27 mg/mL (195.41 mM)
Water 27 mg/mL (195.41 mM)
Ethanol 27 mg/mL (195.41 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Pentylenetetrazol (Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ) displays activity as a central nervous system and respiratory stimulant. It is considered a non-competitive GABA(A) receptor antagonist.
Targets
GABA receptor [1]
In vivo Administration of PTZ (45 mg/kg i.p.) induces clonic convulsions in almost all of the animals after a single injection, typically within 3 min. However, 11% of animals need a second injection to cause clonus. Each rat shows jerking or myoclonus followed by clonus or tonic-clonic convulsions. PTZ injection results in significant changes in GABAA receptor subunit mRNAs. A single PTZ-induced convulsion is associated with rapid changes in GABAA receptors[1].

Protocol (from reference)

Cell Assay:[2]
  • Cell lines

    primary cortical neurons isolated from E16 mice embryos

  • Concentrations

    0, 7.5, 15, 30, and 60 mM

  • Incubation Time

    24 h

  • Method

    Primary cortical neurons are obtained from E16 mice embryos and planted in poly-l-lysine and Laminin coated culturing ware in neurobasal medium with 2% B-27 supplement, 0.5% GlutaMAX-ITM supplement, and 1% penicillin-streptomycin. The culture medium is half replaced every 3 days. The primary cells are cultured for 7 days in vitro (DIV) prior to treating them with isometric fresh medium, PTZ (30 mM), and PTZ (30 mM) + OtoN of different dosages.
Animal Study:[1]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    45 mg/kg

  • Administration

    i.p.

Selleck's Pentylenetetrazol has been cited by 1 publication

MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway [ Cell Mol Life Sci, 2024, 81(1):104] PubMed: 38411738

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.