OSS_128167

Catalog No.S8627 Batch:S862702

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Technical Data

Formula

C19H14N2O6
Molecular Weight 366.32 CAS No. 887686-02-4
Solubility (25°C)* In vitro DMSO 73 mg/mL (199.27 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 3.650mg/ml (9.96mM) Taking the 1 mL working solution as an example, add 50 μL of 73 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.600mg/ml (1.64mM) Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description OSS_128167 (SIRT6-IN-1) is a specific SIRT6 inhibitor with IC50 values of 89, 1578 and 751 μM for SIRT6, SIRT1 and SIRT2, respectively. This compound has anti-viral effect in HBV transcription and replication.
Targets
SIRT6 [1]
(Cell-free)		 SIRT2 [1]
(Cell-free)		 SIRT1 [1]
(Cell-free)
89 μM 751 μM 1578 μM
In vitro OSS-128167 is a selective compounds toward SIRT6 with IC50 values toward SIRT1 and SIRT2 being approximately 20 times higher. It is cell permeable and active in cultured cells. This compound increases H3K9 acetylation and GLUT-1 expression.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    BxPC3 cells

  • Concentrations

    100 μM

  • Incubation Time

    0, 2, 6, 18, 24 h

  • Method

    4 × 105 BxPC3 cells are plated in six-well plates and allowed to adhere for 24 h. Thereafter, cells are stimulated with 100 μM OSS-128167 or the respective amounts of vehicle DMSO for the indicated time amounts. Finally, cells are used for protein lysate generation, and total H3 and acetylated H3K9 levels are detected by immunoblotting.

References

  • https://pubmed.ncbi.nlm.nih.gov/24785705/

Selleck's OSS_128167 Has Been Cited by 22 Publications

Metformin alleviates HFD-induced oxidative stress in hepatocyte via activating SIRT6/PGC-1α/ENDOG signaling [ Clinical Science, November 30, 2022, 1711-1730] PubMed: 36315407
SIRT6-specific inhibitor OSS-128167 exacerbates diabetic cardiomyopathy by aggravating inflammation and oxidative stress [ Molecular Medicine Reports, May 2021, 367] PubMed: 33760202
SIRT6 Improves Hippocampal Neurogenesis Following Prolonged Sleep Deprivation Through Modulating Energy Metabolism in Developing rats [ Molecular Neurobiology, February 2024, 883-899] PubMed: 37668962
Downregulation of Sirt6 by CD38 promotes cell senescence and aging [ Aging (Albany NY), December 6, 2022, 9730-9757] PubMed: 36490326
Sirtuin 6 inhibits group 3 innate lymphoid cell function and gut immunity by suppressing IL-22 production [ Front Immunol, 2024, 15:1402834] PubMed: 39253083
[Gly14]-Humanin ameliorates high glucose-induced endothelial senescence via SIRT6 [ Sci Rep, 2024, 14(1):30924] PubMed: 39730568
Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery [ Antioxidants (Basel), 2023, 12(3)714] PubMed: 36978961
Polydatin Attenuates Cisplatin-Induced Acute Kidney Injury via SIRT6-Mediated Autophagy Activation [ Oxid Med Cell Longev, 2022, 2022:9035547] PubMed: 36160707
SIRT6 regulates obesity-induced oxidative stress via ENDOG/SOD2 signaling in the heart [ Cell Biol Toxicol, 2022, 10.1007/s10565-022-09735-z] PubMed: 35798905
Valdecoxib attenuates lipid-induced hepatic steatosis through autophagy-mediated suppression of endoplasmic reticulum stress [ Biochem Pharmacol, 2022, 199:115022] PubMed: 35358477

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.