NSC45586

Catalog No.S4451 Batch:S445101

Technical Data

Formula

C₂₀H₁₇N₆NaO₃

Molecular Weight

412.38

CAS No.

6300-44-3

Solubility (25°C)*

In vitro

DMSO

82 mg/mL (198.84 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.1mg/ml (9.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 82 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.59mg/ml (1.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 11.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

NSC45586 is an inhibitor of PHLPP (Pleckstrin homology domain and leucine-rich repeat protein phosphatase), which is selective for PHLPP1 and PHLPP2.

Targets

PHLPP ^[1]

In vitro

PH domain and leucine rich repeat phosphatase (Phlpp) inhibitor NSC45586 increases Pth1r mRNA and protein expression in ATDC5 cells and IMCs within 24 hours. ^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines ATDC5 cells, Primary immature chondrocytes (IMCs) Concentrations 25 μM Incubation Time 24 h Method ATDC5 cells were seeded at a density of 5 x 105 cells/well in a six-well plate and incubated overnight to allow for cell adhesion in DMEM supplemented with 10% FBS and 100 units/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL Amphotericin B (1% antibiotic/antimycotic). Phlppi including NSC45586 (25 μM) treatments were added. Primary chondrocytes were plated and allowed to adhere overnight as described above. Actinomycin D (5μM) was added for six hours and then replaced with medium containing Phlppi including this compound or vehicle for 24 hours. Cells were collected for RNA or protein extraction.

Cell Assay:^{^[2]}

Cell lines
ATDC5 cells, Primary immature chondrocytes (IMCs)
Concentrations
25 μM
Incubation Time
24 h
Method
ATDC5 cells were seeded at a density of 5 x 105 cells/well in a six-well plate and incubated overnight to allow for cell adhesion in DMEM supplemented with 10% FBS and 100 units/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL Amphotericin B (1% antibiotic/antimycotic). Phlppi including NSC45586 (25 μM) treatments were added. Primary chondrocytes were plated and allowed to adhere overnight as described above. Actinomycin D (5μM) was added for six hours and then replaced with medium containing Phlppi including this compound or vehicle for 24 hours. Cells were collected for RNA or protein extraction.

References

https://pubmed.ncbi.nlm.nih.gov/24023368/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131217/

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SHIPPING AND STORAGE
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