Nigericin sodium salt

Catalog No.S6653 Batch:S6653

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Technical Data

Formula

C40H67NaO11
Molecular Weight 746.94 CAS No. 28643-80-3
Solubility (25°C)* In vitro Ethanol 100 mg/mL (133.87 mM)
DMSO Insoluble
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
Targets
Potassium channel [1] Hydrogen channel [1] Pb 2+ [1] NLRP3 [2]
In vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H+, K+, and Pb2+ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade .

Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death.

In vivo

Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    H460 cells

  • Concentrations

    1 µM

  • Incubation Time

    24 h

  • Method

    H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).
Animal Study:

[3]
  • Animal Models

    Male C57BL/6 mice

  • Dosages

    4 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/28351327-nigericin-decreases-the-viability-of-multidrug-resistant-cancer-cells-and-lung-tumorspheres-and-potentiates-the-effects-of-cardiac-glycosides/?from_single_result=Nigericin+decreases+the+viability+of+multidrug-resistant+cancer+cells+and+lung+tumorspheres+and+potentiates+the+effects+of+cardiac+glycosides
  • https://pubmed.ncbi.nlm.nih.gov/24089192-mitochondrial-reactive-oxygen-species-induces-nlrp3-dependent-lysosomal-damage-and-inflammasome-activation/?from_single_result=Mitochondrial+ROS+induces+NLRP3-dependent+lysosomal+damage+and+inflammasome+activation
  • https://pubmed.ncbi.nlm.nih.gov/35462332/

Selleck's Nigericin sodium salt Has Been Cited by 43 Publications

2′,4′-dihydroxychalcone alleviates inflammatory bowel disease by inhibiting NLRP3 inflammasome and modulating gut microbiota [ Frontiers in Immunology, 2026, 1751218] PubMed: 41727437
NRG1 Suppresses NLRP3 Inflammasome Activation and Endothelial-Mesenchymal Transition in Cerebral Ischemia-Reperfusion Injury: Association with the AKT/NF-κB Pathway [ Journal of Inflammation Research, 2026, 1-13] PubMed: 41798642
NINJ1 regulates plasma membrane fragility under mechanical strain [ Nature, 2025, 10.1038/s41586-025-09222-5] PubMed: 40490006
Hepatocyte-Specific GSDMD Deficiency Aggravates Sepsis by Disrupting Non-Canonical Secretion of Anti-Inflammatory Factors [ Adv Sci (Weinh), 2025, 12(43):e10412] PubMed: 40856013
PM2.5 exacerbates nasal epithelial barrier dysfunction in allergic rhinitis by inducing NLRP3-mediated pyroptosis via the AhR/CYP1A1/ROS axis [ J Hazard Mater, 2025, 492:138145] PubMed: 40209413
Deacetylation of ATG16L1 is required for LC3-associated lysosomal microautophagy [ Autophagy, 2025, 2948-2962] PubMed: 40851277
ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repair [ Cell Death Dis, 2025, 16(1):681] PubMed: 41053117
Targeting RPLP2 Triggers DLBCL Ferroptosis by Decreasing FXN Expression [ Biomedicines, 2025, 13(6)1320] PubMed: 40564039
GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC [ Mediators Inflamm, 2025, 2025:5576056] PubMed: 40862294
Human cytomegalovirus protein US18 promotes NLRP3 inflammasome activation [ Biochem Biophys Res Commun, 2025, 775:152110] PubMed: 40450990

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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