Acetylcysteine (N-Acetylcysteine, NAC)

Catalog No.S1623 Batch:S162307

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Technical Data

Formula

C5H9NO3S
Molecular Weight 163.19 CAS No. 616-91-1
Solubility (25°C)* In vitro Water 98 mg/mL (600.52 mM)
DMSO 33 mg/mL (202.21 mM)
Ethanol 33 mg/mL (202.21 mM)
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Acetylcysteine (N-acetyl-l-cysteine, NAC, N-acetylcysteine) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor, suppresses TNF-induced NF-κB activation through inhibition of IκB kinases, induces apoptosis via the mitochondria-dependent pathway, and inhibits ferroptosis and virus replication.Solutions are unstable and should be fresh-prepared.
Targets
NF-κB [8] Ferroptosis [9] ROS [6] TNF-α [7]
In vitro

Acetylcysteine (N-Acetylcysteine, NAC) inhibits activation of c-Jun N-terminal kinase, p38 MAP kinase and redox-sensitive activating protein-1 and nuclear factor kappa B transcription factor activities regulating expression of numerous genes. It can also prevent apoptosis and promote cell survival by activating extracellular signal-regulated kinase pathway, a concept useful for treating certain degenerative diseases. This compound directly modifies the activity of several proteins by its reducing activity. [1]

It prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. It also prevents death of PC12 cells and sympathetic neurons. [2]

It causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells. [3]

It activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. This compound protects neuronal cells from death evoked by withdrawal of trophic support. It increases nitric oxide (NO) release from protein-bound stores in vascular tissue. Its pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it was suggested that NAC interferes with redox-sensitive steps in the NGF mechanism. [4]
In vivo

In both the T-maze footshock avoidance paradigm and the lever press appetitive task, N-acetylcysteine (NAC) improves cognition of 12-month-old SAMP8 mice without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight. [5]

Protocol (from reference)

Cell Assay:

[10]
  • Cell lines

    MCF cells

  • Concentrations

    5 mM

  • Incubation Time

    24 h

  • Method

    Cells were treated with the indicated concentration of Acetylcysteine (N-Acetylcysteine, NAC) for 24 h.
Animal Study:

[5]
  • Animal Models

    Male SAMP8 mice

  • Dosages

    100 mg/kg

  • Administration

    s.c.

References

  • https://pubmed.ncbi.nlm.nih.gov/11897584/
  • https://pubmed.ncbi.nlm.nih.gov/7722634/
  • https://pubmed.ncbi.nlm.nih.gov/8631978/
  • https://pubmed.ncbi.nlm.nih.gov/9592085/
  • https://pubmed.ncbi.nlm.nih.gov/12603840/
  • https://pubmed.ncbi.nlm.nih.gov/23772801/
  • https://pubmed.ncbi.nlm.nih.gov/1544168/
  • https://pubmed.ncbi.nlm.nih.gov/29426002/
  • https://pubmed.ncbi.nlm.nih.gov/30294906/
  • https://pubmed.ncbi.nlm.nih.gov/35023619/

Customer Product Validation

(C) Double immunolabeling showed CK-labeled cells (green) co-expressed with 40, 6-diamidino-2-phenylindole (DAPI) (blue), scale bars are 50 μm.

Data from [ , , Biochem Pharmacol, 2017, 146:224-232 ]

Selleck's Acetylcysteine (N-Acetylcysteine, NAC) Has Been Cited by 259 Publications

The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer [ Signal Transduct Target Ther, 2025, 10(1):341] PubMed: 41083431
Inhibition of DEK restores hematopoietic stem cell function in Fanconi anemia [ J Exp Med, 2025, 222(3)e20241248] PubMed: 39836085
FBXO2 Alleviates Intervertebral Disc Degeneration via Dual Mechanisms: Activating PINK1-Parkin Mitophagy and Ubiquitinating LCN2 to Suppress Ferroptosis [ Adv Sci (Weinh), 2025, 12(36):e06150] PubMed: 40791152
PM2.5 exacerbates nasal epithelial barrier dysfunction in allergic rhinitis by inducing NLRP3-mediated pyroptosis via the AhR/CYP1A1/ROS axis [ J Hazard Mater, 2025, 492:138145] PubMed: 40209413
NCOA4-dependent ferritinophagy drives perfluorooctanoic acid-triggered renal ferroptosis in chicken embryos [ J Hazard Mater, 2025, 499:140112] PubMed: 41109030
C-FOS promotes the formation of neutrophil extracellular traps and the recruitment of neutrophils in lung metastasis of triple-negative breast cancer [ J Exp Clin Cancer Res, 2025, 44(1):108] PubMed: 40148973
Multi-omics analysis of glycolytic reprogramming and ROS dynamics in host-specific responses to Salmonella Typhi infection in mice [ J Adv Res, 2025, S2090-1232(25)00345-5] PubMed: 40393644
Targeting PRDX1 impairs acute myeloid leukemic blasts and stem cells by disrupting redox homeostasis [ Cell Death Dis, 2025, 16(1):627] PubMed: 40825764
Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment [ Cell Death Dis, 2025, 16(1):42] PubMed: 39863613
The antitumor effects of lupenone on colon cancer and its mechanistic insights [ Phytomedicine, 2025, 145:156939] PubMed: 40669211

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.