Mitochondrial Fusion Promoter M1

Catalog No.S3375 Batch:S337501

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Technical Data

Formula

C14H10Cl4N2O
Molecular Weight 364.05 CAS No. 219315-22-7
Solubility (25°C)* In vitro DMSO 73 mg/mL (200.52 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Mitochondrial fusion promoter M1 is a mitochondrial dynamic modulator that increases the expression of mitofusin 2, a mitochondrial outer membrane fusion protein, reduces brain mitochondrial dysfunction. Mitochondrial fusion promoter M1 alleviates brain damage in rats with cardiac ischemia/reperfusion injury.
In vitro

Mitochondrial Fusion Promoter M1 prevents the cholesterol mediated blunting of cellular respiration and potentiates Glucose stimulated insulin secretion which is abolished in pancreatic beta cells on cholesterol accumulation.[2]
In vivo

Pretreatment with Mitochondrial Fusion Promoter M1 effectively increases the expression of mitofusin 2, a mitochondrial outer membrane fusion protein, reduces brain mitochondrial dysfunction, BBB breakdown, macrophage infiltration, apoptosis, and AD-related proteins in rats following cardiac I/R injury.[1]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    BRIN-BD11 cells

  • Concentrations

    20 μM

  • Incubation Time

    12 h

  • Method

    BRIN-BD11 cells are first treated with Mitochondrial Fusion Promoter M1 for 12 h and then cholesterol (160 μM) is added to the media for another 12 h before various assays.

Selleck's Mitochondrial Fusion Promoter M1 has been cited by 2 publications

SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics [ Free Radic Biol Med, 2023, 195:270-282] PubMed: 36596388
The effect of mitochondrial fusion on chondrogenic differentiation of cartilage progenitor/stem cells via Notch2 signal pathway [ Stem Cell Res Ther, 2022, 13(1):127] PubMed: 35337368

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.