Imeglimin (EMD 387008) Hydrochloride

Catalog No.S9925 Batch:S992501

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Technical Data

Formula

C6H13N5.ClH

Molecular Weight 191.66 CAS No. 775351-61-6
Solubility (25°C)* In vitro DMSO 38 mg/mL (198.26 mM)
Water 38 mg/mL (198.26 mM)
Ethanol 38 mg/mL (198.26 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Imeglimin (EMD 387008, IMEG, PXL 008, RVT-1501) Hydrochloride is a new class of oral glucose-lowering agents. Imeglimin normalizes glucose tolerance and insulin sensitivity by preserving mitochondrial function from oxidative stress and favoring lipid oxidation in liver of HFHSD mice.

In vitro

Preincubation with Imeglimin (10 mM for 4 h or 100 μM for 24 h) fully prevents tert-butylhydroperoxide (tBH)-induced cell death.<sup><a class="sref" href="#s_ref">[2]</a></sup>

In vivo

Imeglimin normalizes glucose tolerance and insulin sensitivity by preserving mitochondrial function from oxidative stress and favoring lipid oxidation in liver of high-fat, high-sucrose diet (HFHSD) mice.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Assay:

<sup><a class="sref" href="#s_ref">[2]</a></sup>

  • Cell lines

    Human endothelial cells (HMEC-1)

  • Concentrations

    100 μM, 10 mM

  • Incubation Time

    24 h, 4 h

  • Method

    HMEC-1 preincubated with or without Imeglimin (10 mM for 4 h or 100 μM for 24 h) or with CsA (1 μM for 30 min) are ished with PBS before subsequent exposure to 0.5 mM tBH in FBS-free culture medium for 45 min. Cells are then ished with PBS and incubated at 37 °C for 24 h in a complete MCDB medium. Alternatively, cells are exposed to 5.5 mM glucose (control cells) or to 33 mM glucose for 48 h. Cytotoxicity is evaluated with a double-stain system using the Annexin V-Fluoprobes 488 kit combined with propidium iodide.

Animal Study:

<sup><a class="sref" href="#s_ref">[1]</a></sup>

  • Animal Models

    16-week high-fat, high-sucrose diet (HFHSD) mice

  • Dosages

    200 mg/kg

  • Administration

    o.g.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.