HC-030031

Catalog No.S2918 Batch:S291804

Print

Technical Data

Formula

C18H21N5O3
Molecular Weight 355.39 CAS No. 349085-38-7
Solubility (25°C)* In vitro DMSO 71 mg/mL (199.78 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension
0.5% methylcellulose

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 10.000mg/ml (28.14mM) Taking the 1 mL working solution as an example, take 10 mg of this product, add it to 1 ml of 0.5% methylcellulose clear solution, and mix evenly to form a uniform suspension. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description HC-030031 (TOSLAB 829227) is a selective TRPA1 channel blocker that antagonizes AITC- and formalin-evoked calcium influx with IC50 of 6.2 μM and 5.3 μM respectively.
Targets
TRPA1 [1]
In vitro HC-030031 is a substituted theophylline derivative. This compound is a potent and selective TRPA1 inhibitor. It can block both inward and outward currents elicited by AITC or formalin rapidly and reversibly and also blocks the activation of TRPA1 by N-methylmaleimide and by electrophillic prostaglandins. It does not block currents mediated by TRPV1, TRPV3, TRPV4 hERG, or NaV1.2 channels.
In vivo HC-030031 can be delivered to animals orally, by inhalation, or by injection. Oral administration (100 mg/kg) of this compound significantly reversed mechanical hypersensitivity in rat models of chronic inflammatory or neuropathic pain, while local injection (100 μg) into inflamed mouse hind paws attenuated mechanical, but not heat, hypersensitivity.

Protocol (from reference)

Animal Study:[2]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    100, 300 mg/kg

  • Administration

    p.o.

References

  • https://pubmed.ncbi.nlm.nih.gov/17686976/
  • https://pubmed.ncbi.nlm.nih.gov/18954467/
  • https://pubmed.ncbi.nlm.nih.gov/22927999/

Selleck's HC-030031 Has Been Cited by 14 Publications

Rubiadin Mediates the Upregulation of Hepatic Hepcidin and Alleviates Iron Overload via BMP6/SMAD1/5/9-Signaling Pathway [ Int J Mol Sci, 2025, 26(3)1385] PubMed: 39941155
Curcumin suppresses cell proliferation and reduces cholesterol absorption in Caco-2 cells by activating the TRPA1 channel [ Lipids Health Dis, 2023, 22(1):6] PubMed: 36641489
Curcumin suppresses cell proliferation and reduces cholesterol absorption in Caco-2 cells by activating the TRPA1 channel [ Lipids in Health and Disease, 2023, 6] PubMed: 36641489
Toll-like receptor 4 signaling pathway in sensory neurons mediates remifentanil-induced postoperative hyperalgesia via transient receptor potential ankyrin 1 [ Mol Pain, 2023, 19:17448069231158290] PubMed: 36733260
Toll-like receptor 4 signaling pathway in sensory neurons mediates remifentanil-induced postoperative hyperalgesia via transient receptor potential ankyrin 1 [ Molecular Pain, 2023, 17448069231158290] PubMed: 36733260
Berberine Alleviates Gastroesophageal Reflux-Induced Airway Hyperresponsiveness in a Transient Receptor Potential A1-Dependent Manner [ Evidence-Based Complementary and Alternative Medicine, 2022 May 9, 7464147]
Potential Common Mechanisms of Cytotoxicity Induced by Amide Herbicides via TRPA1 Channel Activation [ International Journal of Environmental Research and Public Health, 2022 Jun 29, 7985] PubMed: 35805655
Potential Common Mechanisms of Cytotoxicity Induced by Amide Herbicides via TRPA1 Channel Activation [ International Journal of Environmental Research and Public Health, 2022, 7985] PubMed: 35805655
Potential Common Mechanisms of Cytotoxicity Induced by Amide Herbicides via TRPA1 Channel Activation [ Int J Environ Res Public Health, 2022, 19(13)7985] PubMed: 35805655
Berberine Alleviates Gastroesophageal Reflux-Induced Airway Hyperresponsiveness in a Transient Receptor Potential A1-Dependent Manner [ Evid Based Complement Alternat Med, 2022, 2022:7464147] PubMed: 35586690

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.