Guaiazulene

Catalog No.S3995 Batch:S399501

Technical Data

Formula	C₁₅H₁₈
Molecular Weight	198.30	CAS No.	489-84-9
Solubility (25°C)*	In vitro	DMSO	39 mg/mL (196.67 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Guaiazulene (Azulon, Vetivazulen) is a bicyclic sesquiterpene that is a constituent of some essential oils, mainly oil of guaiac and chamomile oil, which also serve as its commercial sources.
In vitro	Guaiazulene has in vitro cytotoxic activity against neuron and N2a-NB cells^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines N2a-NB cells Concentrations 10, 25, 50, 75, 100, 150, 200 and 400 mg/L Incubation Time 24 h Method The cells were seeded in 96-well plastic plates at a density of 1×10⁴ cells/well and incubated in a humidified atmosphere of 5% CO2 at 37°C for 24 h. Cell viability assay was performed using the MTT cell proliferation kit. Pure water was added in the control group. At the end of the experiment, the neurons, and N2a-NB cells were incubated with 20 µL of MTT for 30 min at 37°C. After washing the blue formazan was extracted from cells with isopropanol/formic acid (955)and was photometrically determined at 570 nm.

Cell Assay:^{^[1]}

Cell lines
N2a-NB cells
Concentrations
10, 25, 50, 75, 100, 150, 200 and 400 mg/L
Incubation Time
24 h
Method
The cells were seeded in 96-well plastic plates at a density of 1×10⁴ cells/well and incubated in a humidified atmosphere of 5% CO2 at 37°C for 24 h. Cell viability assay was performed using the MTT cell proliferation kit. Pure water was added in the control group. At the end of the experiment, the neurons, and N2a-NB cells were incubated with 20 µL of MTT for 30 min at 37°C. After washing the blue formazan was extracted from cells with isopropanol/formic acid (955)and was photometrically determined at 570 nm.

References

[1] Togar B, et al. J Intercult Ethnopharmacol. 2015, 4(1):29-33.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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