Fendiline hydrochloride

Catalog No.S5279 Batch:S527901

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Technical Data

Formula

C23H25N.HCl
Molecular Weight 351.91 CAS No. 13636-18-5
Solubility (25°C)* In vitro DMSO 70 mg/mL (198.91 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Fendiline hydrochloride is the hydrochloride salt form of Fendiline, which is an L-type calcium channel blocker and also a specific inhibitor of K-Ras plasma membrane targeting with no detectable effect on the localization of H- and N-Ras.
Targets
K-ras [1] L-type calcium channel [1]
In vitro Fendiline, an L-type calcium channel blocker, is a specific inhibitor of K-Ras plasma membrane targeting with no detectable effect on the localization of H- and N-Ras. Fendiline does not inhibit K-Ras posttranslational processing but significantly reduced nanoclustering of K-Ras and redistributed K-Ras from the plasma membrane to the endoplasmic reticulum (ER), Golgi apparatus, endosomes, and cytosol. Fendiline significantly inhibited signaling downstream of constitutively active K-Ras and endogenous K-Ras signaling in cells transformed by oncogenic H-Ras. Consistent with these effects, fendiline blocked the proliferation of pancreatic, colon, lung, and endometrial cancer cell lines expressing oncogenic mutant K-Ras[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    MDCK cells

  • Concentrations

    --

  • Incubation Time

    48 h

  • Method

    MDCK cells stably expressing GFP-K-RasG12V were treated with 1% vehicle (DMSO) or various concentrations of fendiline for 48 h. Cells were trypsinized and resuspended in medium. Ten microliters of cell suspension was mixed with 10 μl of 0.4% trypan blue, and live/dead cell counts were obtained using Countess.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.