Ethosuximide

Catalog No.S4626 Batch:S462604

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Technical Data

Formula

C7H11NO2
Molecular Weight 141.17 CAS No. 77-67-8
Solubility (25°C)* In vitro Water 100 mg/mL (708.36 mM)
DMSO 28 mg/mL (198.34 mM)
Ethanol 28 mg/mL (198.34 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Ethosuximide(Zarontin) is a succinimide anticonvulsant, used mainly in absence seizures; A calcium channel blocker.
In vitro

Ethosuximide(ETH) enhances hippocampal derived NSC proliferation and neurospheres formation in vitro. Low concentration of ETH induces proliferation of NSC, while higher concentrations of ETH are cytotoxic. Also, ETH activates the PI3K/Akt signal transduction pathway in adult hippocampal NSC in vitro. Blockade of the PI3K/Akt pathway inhibits ETH induced hippocampal NSC neuronal differentiation[3].
In vivo

Anti-epileptic drug ethosuximide rescues the short lifespan and chemosensory defects exhibited by C. elegans null mutants of dnj-14, the worm orthologue of the DNAJC5 gene mutated in autosomal-dominant adult-onset neuronal ceroid lipofuscinosis. It also ameliorates the locomotion impairment and short lifespan of worms expressing a human Tau mutant that causes frontotemporal dementia[1]. Ethosuximide extends lifespan by inhibiting the function of specific chemosensory neurons[2]. It increases neurogenesis, reduces neurodegeneration, and reverses cognitive impairments in rat model of AD like phenotypes[3].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Mouse neuroblastoma cell line (N2A)

  • Concentrations

    0.1, 0.56, 1 mg/ml

  • Incubation Time

    5 h

  • Method

    Mouse Neuro2A (N2A) neuroblastoma cells are cultured on 6-well plates and treated with retinoic acid to induce neuronal differentiation. After 24 h, N2A cells are treated with vehicle control (PBS) or increasing concentrations of ethosuximide for 5 h. Total RNA is then isolated, DNase-treated and reverse transcribed to cDNA. qRT-PCR is run above normalising to the reference genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin (ACTB).
Animal Study:

[3]
  • Animal Models

    Adult Wistar rats

  • Dosages

    125 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , Nature, 2018, 554(7692):317-322]

Selleck's Ethosuximide has been cited by 1 publication

Ketamine blocks bursting in the lateral habenula to rapidly relieve depression. [Yang Y, et al. Nature, 2018, 554(7692):317-322] PubMed: 29446381

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.