Eribulin Mesylate

Catalog No.S8912 Batch:S891201

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Technical Data

Formula

C41H63NO14S
Molecular Weight 826 CAS No. 441045-17-6
Solubility (25°C)* In vitro DMSO 100 mg/mL (121.06 mM)
Ethanol 100 mg/mL (121.06 mM)
 
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Eribulin Mesylate, an analogue of the marine natural product halichondrin B, is a nontaxane completely synthetic microtubule inhibitor.
Targets
microtubule [1]
In vitro

Eribulin Mesylate (1-100 nM; 72 h) inhibits cells proliferation of LM8 and Dunn cells. Eribulin Mesylate (10-50 nM; 12-72 h) increases early apoptosis significantly in LM8 cells. Eribulin Mesylate (10-50 nM; 12-72 h) induces G2/M arrest by 12 h treatment with at the dose of 50 nM, but not by long-term treatment (72 h) with 10 nM in LM8 cells. Eribulin Mesylate (1-10 nM; 16 h) induces morphological change and suppresses cell migration in a low concentration in LM8 cells.<sup><a class="sref" href="#s_ref">[2]</a></sup>
In vivo

Eribulin Mesylate reduces primary tumor growth and lung metastasis of osteosarcoma in mice.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    LM8 cells, Dunn cells

  • Concentrations

    10 nM, 50 nM

  • Incubation Time

    72 h

  • Method

    LM8 cells and  Dunn cells are incubated with 0 nM, 10 nM, or 50 nM eribulin Mesylate for 12 h, 24 h, 48 h, or 72 h. And then cell proliferation, cell cycle and apoptosis are detected by flow cytometry analysis and immunofluorescence.
Animal Study:

[2]
  • Animal Models

    4-week-old C3H/HeN mice inoculated with LM8 cells

  • Dosages

    1 mg/kg

  • Administration

    I.v.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.