Dimercaprol

Catalog No.S4446 Batch:S444601

Print

Technical Data

Formula

C3H8OS2

Molecular Weight 124.22 CAS No. 59-52-9
Solubility (25°C)* In vitro
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description Dimercaprol (Bal, 2,3-Dimercapto-1-propanol, Dithioglycerol) is an acrolein scavenger that inhibits HIV-1 tat activity, viral production, and infectivity in vitro.
Targets
HIV-1 tat [1]
In vitro

Dimercaprol exerts its acrolein scavenging capability, significantly protects PC-12 cells from acrolein-mediated cell death in a dose dependent manner.[3]

In vivo

Dimercaprol could significantly reduce acrolein contents in spinal cord tissue following a spinal cord contusion injury in rats.[3]

Density 1.239 g/mL

Protocol (from reference)

Cell Assay:

[3]

  • Cell lines

    PC-12 cells

  • Concentrations

    10, 25, 50 and 100 μM

  • Incubation Time

    4 h

  • Method

    Cell viability was tested by WST-1 ([2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium]) assay. Both dimercaprol and acrolein were dissolved in Hank’s Balanced Salt Solution (HBSS). The final concentration of acrolein was 100 μM. The final concentrations of dimercaprol were 10, 25, 50 and 100 μM. PC-12 cells were seeded in 12-well plates at the concentration of 105 cells/well. After overnight incubation, acrolein was added into each well of acrolein group and treatment groups. Equal volumes of HBSS were added into wells of the negative control group. After incubation for 15 minutes, dimercaprol was added at the desired final concentrations (10, 25, 50 and 100 μM) into wells of treatment groups and equal volume of HBSS was added into other wells. The initial 15-minute incubation in acrolein was designed to mimic pathological conditions by allowing acrolein to enter the cells. WST-1 assay solution was added into every well after incubating 4 h, and then allowed to incubate for an additional 2 h.

Animal Study:

[3]

  • Animal Models

    Male Sprague–Dawley rats of spinal cord contusion injury model

  • Dosages

    5 mg/kg

  • Administration

    i.p

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.