Cycloheximide

Catalog No.S7418 Batch:S741803

Technical Data

Formula

C₁₅H₂₃NO₄

Molecular Weight

281.35

CAS No.

66-81-9

Solubility (25°C)*

In vitro

DMSO

56 mg/mL (199.04 mM)

Ethanol

56 mg/mL (199.04 mM)

Water

15 mg/mL (53.31 mM)

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Cycloheximide, an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy.Cycloheximide (NSC-185) can be used to induce animal models of Dysmnesia.Solutions are unstable and should be fresh-prepared.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
In vitro	Cycloheximide, a protein synthesis inhibitor, reduces GSIV-induced host cell death by attenuating phosphatidylserine exposure and loss of ΔΨm from apoptosis/necrosis.
In vivo	This compound, a protein synthesis inhibitors, acts on memory by altering modulators of memory formation as a secondary consequence of the inhibition of protein synthesis.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines grouper fin cell line (GF-1) Concentrations 0.33 µg/ml Incubation Time 0-5 days Method Monolayers of GF-1 cells (4.0 mL, 10⁵ cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.
Animal Study:^{^[2]}	Animal Models Male ICR mice Dosages 30mg/kg, 60mg/kg,120 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
grouper fin cell line (GF-1)
Concentrations
0.33 µg/ml
Incubation Time
0-5 days
Method
Monolayers of GF-1 cells (4.0 mL, 10⁵ cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.

Animal Study:^{^[2]}

Animal Models
Male ICR mice
Dosages
30mg/kg, 60mg/kg,120 mg/kg
Administration
i.p.

References

https://pubmed.ncbi.nlm.nih.gov/26548846/
https://pubmed.ncbi.nlm.nih.gov/22610049/
https://pubmed.ncbi.nlm.nih.gov/5541758/

https://pubmed.ncbi.nlm.nih.gov/30916348/

+ Expand

Selleck's Cycloheximide Has Been Cited by 549 Publications

Polθ activity modulates sensitivity to standard therapies in DNMT3A-deficient leukemia [ Cell Rep Med, 2026, 7(3):102687]	PubMed: 41850232
UBE2M as a bridge spanning neddylation and cell cycle regulation in colorectal adenocarcinoma [ Exp Mol Med, 2026, 58(2):501-518]	PubMed: 41680469
Targeting KIF23 inhibits cell proliferation and primary chemoresistance in cervical cancer by inactivating the MYH9/MCM2/PCNA pathway [ Clin Transl Med, 2026, 16(4):e70652]	PubMed: 41940421
eEF2K-Mediated Stabilization of PCBP2 Promotes Oncogenic mRNA Programs in Triple-Negative Breast Cancer [ Int J Biol Sci, 2026, 22(7):3714-3730]	PubMed: 42003905
O-GlcNAcylation stabilizes RSK4 by antagonizing GSK3β-mediated phosphorylation to enhance radioresistance in esophageal squamous cell carcinoma [ Int J Biol Sci, 2026, 22(5):2418-2434]	PubMed: 41800247
Deubiquitinase UCH-L1 confers paclitaxel resistance via stabilizing PKM2 to promote glycolysis in triple-negative breast cancer [ Cell Death Dis, 2026, 17(1)261]	PubMed: 41741415
OTUD4 deubiquitination stabilizes EGFR and activates the PI3K/AKT pathway to promote the invasiveness of triple-negative breast cancer [ Cell Death Dis, 2026, 17(1)245]	PubMed: 41730840
Viral action on the auxin signaling repressor IAA16 reveals a conserved negative regulator of plant growth and immunity [ Cell Rep, 2026, 45(3):117034]	PubMed: 41734062
USP16 drives psoriasis progression by deubiquitinating and stabilizing NLRP3 in keratinocytes [ JCI Insight, 2026, 11(5)e193017]	PubMed: 41591834
Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling [ Phytomedicine, 2026, 156:158242]	PubMed: 42070337

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.