CVT-10216

Catalog No.E0355 Batch:E035501

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Technical Data

Formula

C24H19NO7S
Molecular Weight 465.48 CAS No. 1005334-57-5
Solubility (25°C)* In vitro DMSO 93 mg/mL (199.79 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.77mg/ml (1.65mM) Taking the 1 mL working solution as an example, add 50 μL of 15.4 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.465mg/ml (1.00mM) Taking the 1 mL working solution as an example, add 50 μL of 9.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CVT-10216 is a highly selective, reversible inhibitor of ALDH-2 with an IC50 of 29 nM.
Targets
ALDH-2 [1] ALDH-1 [1]
29 nM 1.3 μM
In vitro

CVT-10216 significantly reduces about 50% of aldehyde dehydrogenase (ALDH) activity in OCI-AML3 cells even in the presence of recombinant TGF-β1 or BM-MSCs, sensitizing cells to chemotherapy.[2]
In vivo

CVT-10216, a highly selective, reversible inhibitor of ALDH-2, exhibits both acute and prophylactic inhibitions of repeated alcohol-withdrawal-induced anxiety, which counteracts anxiogenic behavior induced by the benzodiazepine receptor inverse agonist in dose-dependently way.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    OCI-AML3 cells

  • Concentrations

    250, 500 nM, 1, 2 µM

  • Incubation Time

    3 days

  • Method

    OCI-AML3 cells are co-cultured with MSCs (200000 cells/well density) and treated with CVT-10216 for 3 days in the presence or absence of recombinant TGF-β1 (5 ng/mL). ALDH activity is measured by ALDEFLUOR assay using flow cytometry.
Animal Study:

[3]
  • Animal Models

    FH, iP and Sprague Dawley (SD) rats

  • Dosages

    3.75, 7.5, or 15 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/19673742/
  • https://pubmed.ncbi.nlm.nih.gov/33253299/
  • https://pubmed.ncbi.nlm.nih.gov/19747934/

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.