Curcumin analog C1

Catalog No.S6769 Batch:S676901

Technical Data

Formula

C₁₉H₁₈O₃

Molecular Weight

294.34

CAS No.

39777-61-2

Solubility (25°C)*

In vitro

DMSO

30 mg/mL (101.92 mM)

Ethanol

2 mg/mL (6.79 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O

1.5mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Curcumin analog C1 (Curcumin analog Compound C1) is a potent transcription factor EB (TFEB) activator with promise for the prevention or treatment of Alzheimer's disease.

Targets

TFEB ^[1]

In vitro

Curcumin analog C1 specifically binds to TFEB at the N terminus and promotes TFEB nuclear translocation without inhibiting MTOR activity. By activating TFEB, Curcumin analog C1 enhances autophagy and lysosome biogenesis in N2a cells. Curcumin analog C1 (0.2-1 μM) dose-dependently increases the levels of LC3-II and SQSTM1/p62 in N2a cells.^[2]

In vivo

The LD⁵⁰ value of Curcumin analog C1 is 175 mg/kg in the acute toxicity assay. Short-term oral administration of Curcumin analog C1 (low dosage 10 mg/kg and high dosage 25 mg/kg; for 24 h) dose-dependently increases the expression of LC3B-II and TFEB in the liver, frontal cortex and striatum of the brain. Chronic administration of Curcumin analog C1 (10 mg/kg; daily; for 21 days) activates TFEB and enhances autophagy in rat brains. ^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines N2a cells, HeLa cells Concentrations 0.2 μM-1 μM Incubation Time 12 h Method N2a cells are treated with curcumin (Cur, 10 μM) and Curcumin analog C1 (1 μM) for 12 h. Cells are fixed and stained with TFEB antibody and DAPI for showing the nuclear translocation of endogenous TFEB. N2a cells are incubated with Curcumin analog C1 (1 μM) for 12 h. The protein levels of LC3B-II and SQSTM1/p62 are determined by western blotting. N2a and HeLa cells are treated with Curcumin analog C1 (0.5, 1 μM) or sucrose (100 mM) for 12 h. The protein levels of the autophagy marker (LC3B-II), TFEB, and lysosome markers (LAMP1, CTSD) are determined by western blotting.

Cell Assay:^{^[2]}

Cell lines
N2a cells, HeLa cells
Concentrations
0.2 μM-1 μM
Incubation Time
12 h
Method
N2a cells are treated with curcumin (Cur, 10 μM) and Curcumin analog C1 (1 μM) for 12 h. Cells are fixed and stained with TFEB antibody and DAPI for showing the nuclear translocation of endogenous TFEB. N2a cells are incubated with Curcumin analog C1 (1 μM) for 12 h. The protein levels of LC3B-II and SQSTM1/p62 are determined by western blotting. N2a and HeLa cells are treated with Curcumin analog C1 (0.5, 1 μM) or sucrose (100 mM) for 12 h. The protein levels of the autophagy marker (LC3B-II), TFEB, and lysosome markers (LAMP1, CTSD) are determined by western blotting.

References

Selleck's Curcumin analog C1 has been cited by 2 publications

Functional TFEB activation characterizes multiple models of renal cystic disease and loss of polycystin-1 [ Am J Physiol Renal Physiol, 2023, 324(4):F404-F422]	PubMed: 36794754
TFEB-Mediated Lysosomal Restoration Alleviates High Glucose-Induced Cataracts Via Attenuating Oxidative Stress [ Invest Ophthalmol Vis Sci, 2022, 63(6):26]	PubMed: 35758908

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.