CU-CPD107

Catalog No.S9937 Batch:S993701

Print

Technical Data

Formula

C16H21IN2O2
Molecular Weight 400.26 CAS No. 2573912-32-8
Solubility (25°C)* In vitro DMSO 80 mg/mL (199.87 mM)
Ethanol 80 mg/mL (199.87 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 4mg/ml (9.99mM) Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.57mg/ml (1.42mM) Taking the 1 mL working solution as an example, add 50 μL of 11.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CU-CPD107 is a selective, dual-activity small-molecule which demonstrated differential activity against the TLR8 agonists and ssRNA ligands. In the presence of R848, this compound acts as a TLR8 signaling inhibitor (IC50=13.7 μM). In the presence of ssRNA, it shows synergistic agonist activities, while this chemical alone is unable to influence TLR8 signaling.
Targets
TLR8 [1]
(in HEK-Blue hTLR8 cells)
13.7 μM
In vitro

CU-CPD107, a tetrasubstituted imidazole, inhibits R848-induced TLR8 signaling and shows unexpected synergistic agonist activities in the presence of ssRNA, while this compound alone is unable to influence TLR8 signaling.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HEK-Blue cells, HEK-Blue hTLR cells, HEK-Blue Null1 cells

  • Concentrations

    0-100 µM

  • Incubation Time

    24 h

  • Method

    Cellular toxicity was assessed using the Roche Cell Proliferation Reagent WST1 per the manufacturer's recommendation. Briefly, WST1 was diluted 1:10 into cell-containing media and assayed with absorbance at 450 nm.

References

  • https://pubmed.ncbi.nlm.nih.gov/34272380/

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.