CTB

Catalog No.E1107 Batch:E110702

Technical Data

Formula	C₁₆H₁₃ClF₃NO₂
Molecular Weight	343.73	CAS No.	451491-47-7
Solubility (25°C)*	In vitro	DMSO	69 mg/mL (200.73 mM)
		Ethanol	17 mg/mL (49.45 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	CTB is a p300 activator and the lowest docked energy for CTB and p300 is 7.72 kcal/mol.
In vitro	CTB effectively induces apoptosis, up-regulates the expression of P300, and increases the acetylation and total P53 protein levels in MCF-7 cells.^[2]
In vivo	CTB combinated with CpG, and aluminum hydroxide playsa synergistic effect to enhance ClfA_221-550-induced immune responses, which possesses the potential to develop a novel vaccine to prevent infection of S. aureus.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MCF-7 and MRC-5 cells Concentrations 85.43 µM Incubation Time 24, 48, 72 h Method MCF-7 and MRC-5 are cultured in RPMI-1640 and treated with or without CTB at a concentration of 85.43 μmol/L at different times (24, 48 and 72 h). The percentage of apoptotic cells are measured by flow cytometry.Real-time quantitative RT-PCR is performed to estimate the mRNA expression of P300 in MCF-7 and MRC-5 with CTB at different times. ELISA and Bradford protein techniques are used to detect levels of total and acetylated P53 protein generated in MCF-7 and MRC-5.
Animal Study:^{^[3]}	Animal Models Specific-pathogen-free BALB/c mice Dosages 100 μg Administration i.m.

Cell Assay:^{^[2]}

Cell lines
MCF-7 and MRC-5 cells
Concentrations
85.43 µM
Incubation Time
24, 48, 72 h
Method
MCF-7 and MRC-5 are cultured in RPMI-1640 and treated with or without CTB at a concentration of 85.43 μmol/L at different times (24, 48 and 72 h). The percentage of apoptotic cells are measured by flow cytometry.Real-time quantitative RT-PCR is performed to estimate the mRNA expression of P300 in MCF-7 and MRC-5 with CTB at different times. ELISA and Bradford protein techniques are used to detect levels of total and acetylated P53 protein generated in MCF-7 and MRC-5.

Animal Study:^{^[3]}

Animal Models
Specific-pathogen-free BALB/c mice
Dosages
100 μg
Administration
i.m.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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