CEBPα inducer 1

Catalog No.S3538 Batch:S353801

Technical Data

Formula	C₂₀H₁₂FN₃O₄
Molecular Weight	377.33	CAS No.	2374285-52-4
Solubility (25°C)*	In vitro	DMSO	9 mg/mL (23.85 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

C/EBPα inducer 1 (compound 78) is a potent inducer of CCAAT enhancer-binding protein α (C/EBPα) and myeloid differentiation.

Targets

C/EBPα ^[1]

In vitro

C/EBPα inducer 1 (compound 78) causes differentiation at 3 μM concentration, increases the expression of neutrophil differentiation marker CD11b, and upregulates both the C/EBPα and C/EBPε levels.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HL-60 cells Concentrations 3 μM Incubation Time 7 days Method HL-60 cells were treated with 0.1% DMSO and 3 µM of C/EBPα inducer 1 (compound 78) for 7 days and stained with Wright-Giemsa. Maximum cell concentrations were adjusted lower than 100,000 /mL. These treated HL-60 cells were incubated with 1.0 mg/mL nitroblue tetrazolium/PBS, and 0.36 µM Phorbol-12-myristate-13-acetate (PMA) at 37 °C for 30–40 min and stained with safranin O. Morphological differentiation and apoptosis were evaluated as − (0–5%), + (5–30%), and ++ (more than 30%).

Cell Assay:^{^[1]}

Cell lines
HL-60 cells
Concentrations
3 μM
Incubation Time
7 days
Method
HL-60 cells were treated with 0.1% DMSO and 3 µM of C/EBPα inducer 1 (compound 78) for 7 days and stained with Wright-Giemsa. Maximum cell concentrations were adjusted lower than 100,000 /mL. These treated HL-60 cells were incubated with 1.0 mg/mL nitroblue tetrazolium/PBS, and 0.36 µM Phorbol-12-myristate-13-acetate (PMA) at 37 °C for 30–40 min and stained with safranin O. Morphological differentiation and apoptosis were evaluated as − (0–5%), + (5–30%), and ++ (more than 30%).

References

[1] Radhakrishnan Sridhar, et al. Molecules. 2018 Aug 3;23(8):1938.

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SHIPPING AND STORAGE
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