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Technical Data
| Formula | C6H15N2O2.Cl |
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| Molecular Weight | 182.65 | CAS No. | 51-83-2 | |
| Solubility (25°C)* | In vitro | Water | 36 mg/mL (197.09 mM) | |
| Ethanol | 12 mg/mL (65.69 mM) | |||
| DMSO | 5 mg/mL (27.37 mM) | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Preparing Stock Solutions
Biological Activity
| Description | Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing. |
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| In vitro | Carbachol causes an immediate increase in short circuit current (Isc) that peaked within 5 min and declined rapidly thereafter in T84 cell monolayers with IC50 of 10 mM, although a small increase in Isc persisted for approximately 30 min. Carbachol increases basolateral K+ efflux by activating two different K+ transport pathways on the basolateral membrane in T84 cell monolayers. [1] Carbachol (100 μM) stimulates a time-dependent increase in tyrosine phosphorylation of a range of proteins in T84 cells. Carbachol (100 μM) stimulates an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase in T84 cells. Carbachol (100 μM) stimulates tyrosine phosphorylation of the EGF receptor (EGFr) and increases co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr in T84 cells. [2] Carbachol (3.3 nmol dose) produces a transient increase in NGF mRNA levels present 4-8 hours in hippocampus of rats. Carbachol causes transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend in rats. [3] |
| In vivo | Carbachol (1-10 ng) infused specifically into the posterior oral pontine reticular nucleus (PnO) results in induction of rapid eye movement sleep in the rat. Carbachol (500 ng) does not affect REM sleep but enhances wakefulness in the rat. [4] |
Protocol (from reference)
References
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.