Butylparaben

Catalog No.S4584 Batch:S458401

Technical Data

Formula	C₁₁H₁₄O₃
Molecular Weight	194.23	CAS No.	94-26-8
Solubility (25°C)*	In vitro	DMSO	38 mg/mL (195.64 mM)
		Water	38 mg/mL (195.64 mM)
		Ethanol	38 mg/mL (195.64 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Butylparaben (Butyl parahydroxybenzoate, Butyl 4-hydroxybenzoate) is a chemical compound commonly used as an antifungal preservative in cosmetic products.
In vitro	After butylparaben treatment, vimentin filaments in Sertolicells are disrupted. Sertoli cell cytoskeleton responds to butylparaben by exhibiting a marked collapse in vimentin filaments distribution^[1].
In vivo	Butylparaben induces collapse of Sertoli cell vimentin filaments in vivo^[1]. Exposure of a postweaning mammal to butylparaben had an adverse effect on the secretion of testosterone and in the functions of the male reproductive system^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Primary Sertoli cell Concentrations 0, 1, 100 and 1000 nmol/ml Incubation Time 6, 24h Method Mixed cultures of Sertoli and spermatogenic cells are prepared from three-week-old SD rats. Single cells are obtained by gravity sedimentation and use of 50% trypsin-EDTA. Cells are finally suspended at a concentration of 1 × 10⁸cell/ml and maintained for 24 h in Dulbecco’s modified Eagle medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovineserum. Thereafter, cells are maintained in serum-freemedium, which is changed every 24 h. At 72 h, spermatogenic cells are removed by treatment with hypotonic Tris buffer (20 mMTris–HCl, pH 7.4) for 5 min. The Sertoli cell-enriched cultures obtained are then maintained in serum-free medium for 24 h before being reseeded onto chamber slides. The samples are cultured for a further 5 days. Subsequently, they are exposed to 0, 1, 100 and 1000 nmol/ml butylparaben for 6 and 24 h. Some slides are stained with 0.5% toluidine blue for light microscopic observations, whereas the rest are used for vimentin immuno-histochemical analysis.
Animal Study:^{^[1]}	Animal Models Male Sprague–Dawley rats Dosages 1000 mg/kg Administration oral administration

Cell Assay:^{^[1]}

Cell lines
Primary Sertoli cell
Concentrations
0, 1, 100 and 1000 nmol/ml
Incubation Time
6, 24h
Method
Mixed cultures of Sertoli and spermatogenic cells are prepared from three-week-old SD rats. Single cells are obtained by gravity sedimentation and use of 50% trypsin-EDTA. Cells are finally suspended at a concentration of 1 × 10⁸cell/ml and maintained for 24 h in Dulbecco’s modified Eagle medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovineserum. Thereafter, cells are maintained in serum-freemedium, which is changed every 24 h. At 72 h, spermatogenic cells are removed by treatment with hypotonic Tris buffer (20 mMTris–HCl, pH 7.4) for 5 min. The Sertoli cell-enriched cultures obtained are then maintained in serum-free medium for 24 h before being reseeded onto chamber slides. The samples are cultured for a further 5 days. Subsequently, they are exposed to 0, 1, 100 and 1000 nmol/ml butylparaben for 6 and 24 h. Some slides are stained with 0.5% toluidine blue for light microscopic observations, whereas the rest are used for vimentin immuno-histochemical analysis.

Animal Study:^{^[1]}

Animal Models
Male Sprague–Dawley rats
Dosages
1000 mg/kg
Administration
oral administration

References

Selleck's Butylparaben has been cited by 1 publication

Butyl paraben promotes apoptosis in human trophoblast cells through increased oxidative stress-induced endoplasmic reticulum stress [ Environ Toxicol, 2018, 33(4):436-445]	PubMed: 29319206

Butyl paraben promotes apoptosis in human trophoblast cells through increased oxidative stress-induced endoplasmic reticulum stress [ Environ Toxicol, 2018, 33(4):436-445]

PubMed: 29319206

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.