Bobcat339 hydrochloride

Catalog No.S6682 Batch:S668205

Technical Data

Formula

C₁₆H₁₃Cl₂N₃O

Molecular Weight

334.2

CAS No.

2436747-44-1

Solubility (25°C)*

In vitro

DMSO

67 mg/mL (200.47 mM)

Ethanol

67 mg/mL (200.47 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	0.3mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.35mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 67 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Bobcat339 is a selective cytosine-based TET enzyme inhibitor with IC50 of 33 μM and 73 μM for TET1 and TET2, respectively. Bobcat339 can reduce DNA 5-hydroxymethylcytosine abundance by inhibiting TET enzyme function in living cells and provide support for its utility as a viable pharmacological probe.

Targets

TET1 ^[1] (Cell-free assay)	TET2 ^[1] (Cell-free assay)
33 μM	73 μM

In vitro

Bobcat339, the TET2 enzyme activity inhibitor, decreases the TET2 enzyme activity and protects cells from neurotoxin damage in SH-SY5Y cells.^[2]

In vivo

Bobcat339 hydrochloride is a potent and selective cytosine-based inhibitor of TET enzyme.

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines SH-SY5Y neuroblastoma cells Concentrations 3 μM Incubation Time 12 h Method The CCK-8 assay kit was used to determine cell viability. SH-SY5Y cells are seeded into 96-well plates at a density of 5,000 cells/well with 100 μl of cell medium in each well. Bobcat339 (3 μM) is preincubated with cells for 12 h before MPP+ (2.5 mM) treatment. Then, 10 μl of CCK-8 working solution is added to each well to incubate for about 1 h at 37 C. The wells containing only the culture medium served as blanks. The absorbance value at 450 nm is measured.

Cell Assay:^{^[2]}

Cell lines
SH-SY5Y neuroblastoma cells
Concentrations
3 μM
Incubation Time
12 h
Method
The CCK-8 assay kit was used to determine cell viability. SH-SY5Y cells are seeded into 96-well plates at a density of 5,000 cells/well with 100 μl of cell medium in each well. Bobcat339 (3 μM) is preincubated with cells for 12 h before MPP+ (2.5 mM) treatment. Then, 10 μl of CCK-8 working solution is added to each well to incubate for about 1 h at 37 C. The wells containing only the culture medium served as blanks. The absorbance value at 450 nm is measured.

References

Selleck's Bobcat339 hydrochloride has been cited by 5 publications

Ten-eleven translocation-2-mediated macrophage activation promotes liver regeneration [ Cell Commun Signal, 2024, 22(1):95]	PubMed: 38308318
Association of DNA methylation/demethylation with the functional outcome of stroke in a hyperinflammatory state [ Neural Regeneration Research, 2024, 19(10):p 2229-2239]	PubMed: none
TET Family Members Are Integral to Porcine Oocyte Maturation and Parthenogenetic Pre-Implantation Embryogenesis [ Int J Mol Sci, 2023, 24(15)12455]	PubMed: 37569830
SIRT1 Deacetylates TET2 and Promotes Its Ubiquitination Degradation to Achieve Neuroprotection Against Parkinson's Disease [ Front Neurol, 2021, 12:652882]	PubMed: 33935952
TET2 suppresses nasopharyngeal carcinoma progression by inhibiting glycolysis metabolism [ Cancer Cell Int, 2020, 20:363]	PubMed: 32774157

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.