Bithionol

Catalog No.S4552 Batch:S455201

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Technical Data

Formula

C12H6Cl4O2S
Molecular Weight 356.05 CAS No. 97-18-7
Solubility (25°C)* In vitro DMSO 71 mg/mL (199.41 mM)
Ethanol 22 mg/mL (61.78 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.55mg/ml Taking the 1 mL working solution as an example, add 50 μL of 71 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5% DMSO 95% Corn oil
0.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Bithionol (Actamer) is a potent inhibitor of soluble adenylyl cyclase(sAC) with an IC50 of 4 μM and has antibacterial and anthelmintic properties along with algaecide activity.
Targets
sAC [2]
(Cell-free assay)
4.0 μM

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    ovarian cancer cell lines

  • Concentrations

    0.178 μM-400μM

  • Incubation Time

    48h, 72h

  • Method

    Ovarian cancer cell lines (5 × 103 cells/well) were plated into 96-well flat bottom plates and incubated for overnight. Cells were treated with different concentrations of BT ranging from 0.178 μM to 400 μM and further incubated for 48 hrs or 72 hrs.

Selleck's Bithionol has been cited by 3 publications

Piezo1 induces endothelial responses to shear stress via soluble adenylyl Cyclase-IP3R2 circuit [ iScience, 2023, 10.1016/j.isci.2023.106661] PubMed: 37168565
Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma. [ Cancer Lett, 2020, 479:100-111] PubMed: 32200035
Autotaxin-Lysophosphatidic Acid Axis Blockade Improves Inflammation by Regulating Th17 Cell Differentiation in DSS-Induced Chronic Colitis Mice. [ Inflammation, 2019, 42(5):1530-1541] PubMed: 31102124

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.