Betulin

Catalog No.S4754 Batch:S475402

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Technical Data

Formula

C30H50O2

Molecular Weight 442.72 CAS No. 473-98-3
Solubility (25°C)* In vitro DMSO 5 mg/mL (11.29 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Betulin (BE, betulinol, betuline, betulinic alcohol), also known as betulinol, betuline, or betulinic alcohol, is a pentacyclic lupane-type triterpenoid naturally distributed in many plants and displays a broad spectrum of biological and pharmacological properties such as anticancer and chemopreventive activity. Betulin (Trochol), is a sterol regulatory element-binding protein (SREBP) inhibitor with an IC50 of 14.5 μM in K562 cell line.
In vitro Betulin significantly inhibits cell viability in cervix carcinoma HeLa cells, hepatoma HepG2 cells, lung adenocarcinoma A549 cells, and breast cancer MCF-7 cells with IC50 values ranging from 10 to 15 mg/mL. While betulin exhibits only moderate anticancer activity in other human cancer cells such as hepatoma SK-HEP-1 cells, prostate carcinoma PC-3, and lung carcinoma NCI-H460, with IC50 values ranging from 20 to 60 mg/mL, it shows minor growth inhibition in human erythroleukemia K562 cells (IC50>100 mg/mL). Betulin does not directly trigger mitochondrial cytochrome c release in isolated mitochondria. Bax and Bak are rapidly translocated to the mitochondria 30 min after betulin treatment[1]. In vitro, Betulin inhibits LPS-induced tumor necrosis factor α (TNF-α) and (interleukin) IL-6 levels and up-regulates the level of IL-10. Also Betulin suppresses the phosphorylation of nuclear factor-kB (NF-kB) p65 protein in LPS-stimulated RAW 264.7 cells[2].
In vivo In vivo, Betulin alleviates LPS-induced acute lung injury. Treatment with Betulin diminishes pro-inflammatory cytokines, myeloperoxidase activity and bacterial loads in lung tissue during gramnegative pneumonia. In HFD-fed apoE−/− mice, betulin administration significantly reduces lesions in en face aortas and aortic sinuses. Furthermore, betulin administration significantly increases ABCA1 expression and suppresses macrophage positive areas in the aortic sinuses. Moreover, betulin administration improves plasma lipid profiles and enhances fecal cholesterol excretion in the mice[3].

Protocol (from reference)

Animal Study:[2]
  • Animal Models

    C57BL/6J male mice aged 8 weeks

  • Dosages

    4 or 8 mg/kg

  • Administration

    i.p.

Selleck's Betulin has been cited by 5 publications

SREBP2 inhibitor betulin sensitizes hepatocellular carcinoma to lenvatinib by inhibiting the mTOR/IL-1β pathway [ Acta Biochim Biophys Sin -Shanghai), 2023, 55(9):1479-1486] PubMed: 37434430
SREBP2 inhibitor betulin sensitizes hepatocellular carcinoma to lenvatinib by inhibiting the mTOR/IL-1β pathway [ Acta Biochim Biophys Sin (Shanghai), 2023, 55(9):1479-1486] PubMed: 37434430
Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication [ Nat Metab, 2021, 10.1038/s42255-021-00479-4] PubMed: 34580494
Organ-specific cholesterol metabolic aberration fuels liver metastasis of colorectal cancer [ Theranostics, 2021, 11(13):6560-6572] PubMed: 33995676
Inhibition of SREBP-1 Activation by a Novel Small-Molecule Inhibitor Enhances the Sensitivity of Hepatocellular Carcinoma Tissue to Radiofrequency Ablation [ Front Oncol, 2021, 11:796152] PubMed: 34900747

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.