ATP disodium

Catalog No.S1985 Batch:S198502

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Technical Data

Formula

C10H14N5O13P3.2Na

Molecular Weight 551.14 CAS No. 987-65-5
Solubility (25°C)* In vitro Water 100 mg/mL (181.44 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description ATP disodium is a disodium salt form of adenosine-triphosphate which is a multifunctional nucleoside triphosphate.
In vitro

ATP release and autocrine feedback through P2Y2 and A3 receptors provide signal amplification, controlling gradient sensing and migration of neutrophils. [1] ATP results in production of reactive oxygen species (ROS), which stimulates the phosphatidylinositol 3-kinase (PI3K) pathway and subsequent Akt and ERK1/2 activation. ATP-dependent ROS production and PI3K activation also stimulate transcription of genes required for an oxidative stress response. ATP-mediated ROS-dependent PI3K is required for activation of caspase-1 and secretion of IL-1beta and IL-18. [2] ATP potently stimulates TNF-alpha release, resulting from TNF-alpha mRNA expression in rat cultured brain microglia. ATP-induced TNF-alpha release is Ca(2+)-dependent, and a sustained Ca(2+) influx correlated with the TNF-alpha release in ATP-stimulated microglia. ATP-induced TNF-alpha release is inhibited by PD 098059, an inhibitor of extracellular signal-regulated protein kinase (ERK) kinase 1 (MEK1), which activates ERK, and also by SB 203580, an inhibitor of p38 mitogen-activated protein kinase. ATP rapidly activates both ERK and p38 even in the absence of extracellular Ca(2+). [3] ATP-induced cytotoxicity is mediated by classical alterations of apoptosis, including membrane blebbing, nuclear condensation, and DNA fragmentation. ATP but not other nucleotides lead to the potent and selective activation of NF-κB in microglial cells through a P2Z receptor-mediated pathway. [4]

In vivo

ATP regulates microglial branch dynamics in the intact mice brain, and its release from the damaged tissue and surrounding astrocytes mediates a rapid microglial response towards injury. [5]

Protocol (from reference)

Selleck's ATP disodium has been cited by 17 publications

Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure [ EBioMedicine, 2023, 10.1016/j.ebiom.2023.104863] PubMed: 37950995
Induction of Synthetic Lethality by Activation of Mitochondrial ClpP and Inhibition of HDAC1/2 in Glioblastoma [ Clin Cancer Res, 2022, 28(9):1881-1895] PubMed: 35417530
ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury [ Cell Signal, 2022, 93:110304] PubMed: 35278669
Extracellular phosphoprotein regulation is affected by culture system scale-down [ Biochim Biophys Acta Gen Subj, 2022, 1866(8):130165] PubMed: 35513203
Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines [ Transl Vis Sci Technol, 2022, 11(4):4] PubMed: 35377941
Human TBK1 deficiency leads to autoinflammation driven by TNF-induced cell death [ Cell, 2021, S0092-8674(21)00885-0] PubMed: 34363755
A Semi-automated System of Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of African Swine Fever Virus Antibody [ J Clin Microbiol, 2021, JCM0099021] PubMed: 34260273
Dexmedetomidine Mitigated NLRP3-Mediated Neuroinflammation via the Ubiquitin-Autophagy Pathway to Improve Perioperative Neurocognitive Disorder in Mice [ Front Pharmacol, 2021, 12:646265] PubMed: 34079457
Fos Facilitates Gallid Alpha-Herpesvirus 1 Infection by Transcriptional Control of Host Metabolic Genes and Viral Immediate Early Gene [ Viruses, 2021, 13(6)1110] PubMed: 34207926
GSDMD membrane pore is critical for IL-1β release and antagonizing IL-1β by hepatocyte-specific nanobiologics is a promising therapeutics for murine alcoholic steatohepatitis. [ Biomaterials, 2020, 227:119570] PubMed: 31670032

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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