Amitraz

Catalog No.S3643 Batch:S364301

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Technical Data

Formula

C19H23N3
Molecular Weight 293.41 CAS No. 33089-61-1
Solubility (25°C)* In vitro DMSO 58 mg/mL (197.67 mM)
Ethanol 58 mg/mL (197.67 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Amitraz (NSC 324552) is a triazapentadiene, an α2 adrenergic agonist and a member of the amidine chemical family. It is a non-systemic acaricide and insecticide.
Targets
α2 adrenergic receptor [1]
In vitro Amitraz was not cytotoxic to human luteinized granulosa cells at concentrations of 1, 10 or 50 µg/ml for exposures of 2-72 h While the highest concentration of this compound tested, 100 µg/ml, caused significant cell death after exposures of 24, 48 and 72 h. It decreased the amount of progesterone produced by each cell after a 4 h exposure; the reduction in progesterone concentration was not caused by decreased cell viability.
In vivo Amitraz is an α2-adrenergic receptor (α2-AR) agonist that adversely affects the mammalian reproductive system by binding to presynaptic α2-AR in the hypothalamus, thus inhibiting noradrenalin release and decreasing GnRH secretion. When 30 mg/kg of this compound was administered to rats, the ovulatory LH surge was prevented. It inhibited insulin but stimulated glucagon secretion in a perfused rat pancreas model. This chemical also decreased intestinal motility, and inhibited prostaglandin synthesis by bovine seminal vesicle microsomes. It can be absorbed through the skin and can exert systemic effects via the vascular system in humans. Low doses of AMZ (l-25 mg/kg) decreased motor activity and altered the rates and patterns of responding under schedule-controlled conditions. Intermediate doses (50-100 mg/kg) affect visual-evoked potentials and lower body weight and temperature. In addition, 100 mg/kg produces slight MAO inhibition and characteristic signs of intoxication (e.g., weight loss and hyperreactivity). High doses (>lOO mg/kg) produce more pronounced MAO inhibition, extreme weight loss and hyperreactivity, aggression, and lethality.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Luteinized human granulosa cells

  • Concentrations

    1, 10, 50 and 100 µg/ml

  • Incubation Time

    2, 4, 6, 24, 48 or 72 h

  • Method

    Cells were exposed to 1, 10, 50 and 100 µg/ml amitraz for 2, 4, 6, 24, 48 or 72 h.
Animal Study:

[2]
  • Animal Models

    Adult male Long-Evans hooded rats

  • Dosages

    1-200 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/16085667/
  • https://pubmed.ncbi.nlm.nih.gov/2925011/

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.