AG-636

Catalog No.S9776 Batch:S977601

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Technical Data

Formula

C21H17N3O2

Molecular Weight 343.38 CAS No. 1623416-31-8
Solubility (25°C)* In vitro DMSO 35 mg/mL (101.92 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description AG-636 is an orally available inhibitor of dihydroorotate dehydrogenase (DHODH) with potential antineoplastic activity.
Targets
DHODH [1]
In vitro

AG-636 reveals a strong enrichment in sensitivity to a novel dihydroorotate dehydrogenase (DHODH) in cancer cell lines of hematologic versus solid tumor origin.[1]

In vivo

AG-636 results in robust tumor growth inhibition in xenograft lymphoma tumor models, with comparably poor activity in solid tumor models.[1]

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    Z138 cells, OCILY19 cells, JEKO1 cells, HCT116 cells, A549 cells

  • Concentrations

    10 μM

  • Incubation Time

    96 h

  • Method

    For CTG assays, cells are plated in 96-well tissue culture plates at either 10,000 cells/well for cell lines of hematologic origin or 2000 cells/well for solid tumor-derived cell lines. Cells are incubated with AG-636 or DMSO control for 96 hours. CTG readings are performed at time 0-(T0) and at time 96-hour (T96) using a Molecular Devices SpectraMAX Paradigm plate reader.

Animal Study:

[1]

  • Animal Models

    Transgenic female 6-8-week-old CB17 SCID mice, Female 6-8-week-old BALB/c mice

  • Dosages

    10 mg/kg, 30 mg/kg, 100 mg/kg

  • Administration

    PO

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.