Quinine HCl Dihydrate

Catalog No.S2502 Batch:S250202

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Technical Data

Formula

C20H24N2O2.HCl.2H2O
Molecular Weight 396.91 CAS No. 6119-47-7
Solubility (25°C)* In vitro DMSO 79 mg/mL (199.03 mM)
Ethanol 79 mg/mL (199.03 mM)
Water 43 mg/mL (108.33 mM)
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 3.95mg/ml (9.95mM) Taking the 1 mL working solution as an example, add 50 μL of 79 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5 %DMSO 95 %Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.56mg/ml (1.41mM) Taking the 1 mL working solution as an example, add 50 μL of 11.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Quinine HCl Dihydrate is a white crystalline K+ channel blocker, used to treat malaria.
Targets
Potassium channel [1]
In vitro Quinine blocks Cx36 and Cx50 junctional currents in a reversible and concentration-dependent manner with half maximal blocking concentrations of 32 mM and 73 mM, respectively. Quinine induces slow transitions between open and fully closed states that decreased open probability of the channel. Quinine thus offers a potentially useful method to block certain types of gap junction channels, including those between neurons that are formed by Cx36. Quinine, a K+ channel blocker, prevents formation of tumor necrosis factor (TNF) as well as the subsequent hepatic DNA fragmentation and liver enzyme leakage. Quinine elicits Fos-like immunoreactivity (FLI) concentrated in the medial third of the nucleus; acid elicited more broadly distributed FLI concentrated farther laterally. Quinine has a relatively weak effect on doxorubicin accumulation but was able to completely restore doxorubicin sensitivity in the resistant cells. Quinine also modifies the intracellular tolerance to doxorubicin, which suggests that it is able to modify drug distribution within the cells. Quinine primarily blocks the whole cell potassium currents (IK) in a voltage-dependent manner. Quinine also reduces the size of sodium currents (INa) in a use-dependent manner, while leaving calcium currents (ICa) relatively unaffected.

Protocol (from reference)

References

  • https://pubmed.ncbi.nlm.nih.gov/11535816/
  • https://pubmed.ncbi.nlm.nih.gov/8788453/
  • https://pubmed.ncbi.nlm.nih.gov/12010763/
  • https://pubmed.ncbi.nlm.nih.gov/7628869/
  • https://pubmed.ncbi.nlm.nih.gov/9582223/

Selleck's Quinine HCl Dihydrate Has Been Cited by 2 Publications

Comparative analysis of media effects on human induced pluripotent stem cell-derived cardiomyocytes in proarrhythmia risk assessment [ Journal of Pharmacological and Toxicological Methods, 2017, 39-47] PubMed: nan
Comparative analysis of media effects on human induced pluripotent stem cell-derived cardiomyocytes in proarrhythmia risk assessment [ J Pharmacol Toxicol Methods, 2017, 90:39-47] PubMed: 29155283

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.