Aloxistatin (E64d)

Catalog No.S7393 Batch:S739302

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Technical Data

Formula

C17H30N2O5
Molecular Weight 342.43 CAS No. 88321-09-9
Solubility (25°C)* In vitro DMSO 68 mg/mL (198.58 mM)
Ethanol 68 mg/mL (198.58 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Aloxistatin (E64d) is an irreversible and membrane-permeable cysteine protease inhibitor with blood platelet aggregation inhibiting activity. The cysteine protease cathepsin L is required for SARS-CoV-2 viral entry, and aloxistatin treatment reduced cellular entry of SARS-CoV-2 pseudovirions by 92.3%.
Targets
Cysteine protease [1]
In vitro

Aloxistatin can enter the intact platelet and inhibit proteolysis by inhibiting calpain. [2]

Aloxistatin blunts Parathyroid hormone (PTH)-induced cell proliferation and inhibits differentiation osteoblasts in vitro. [3]
In vivo

Aloxistatin (100 mg/kg, p.o.) strongly inhibits the cathepsin B&L activities in the skeletal muscle, heart and liver of hamsters. [1]

In spinal cord injury (SCI) rats, Aloxistatin provides neuroprotection in SCI lesion and penumbra. [4]

Aloxistatin reduces brain amyloid-β and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B activity. [5]

Protocol (from reference)

Cell Assay:

[6]
  • Cell lines

    NCM460 cells

  • Concentrations

    1 μM

  • Incubation Time

    24 h

  • Method

    Cells were treated with indicated concentration of the drug for 24 hour.
Animal Study:

[1]
  • Animal Models

    Hamsters

  • Dosages

    ~100 mg/kg

  • Administration

    p.o.

Selleck's Aloxistatin (E64d) has been cited by 50 publications

Enzymatic combinatorial synthesis of E-64 and related cysteine protease inhibitors [ Nat Chem Biol, 2025, 10.1038/s41589-025-01907-2] PubMed: 40346252
HLS1 interacts with ATG8 to negatively regulate the ABS3-mediated plant senescence pathway [ Cell Rep, 2025, 44(4):115507] PubMed: 40215167
The phagosome-mediated anti-bacterial immunity is governed by the proton-activated chloride channel in peritoneal macrophages [ bioRxiv, 2025, 2025.02.27.640612] PubMed: 40060571
Truncating NFKB1 variants cause combined NLRP3 inflammasome activation and type I interferon signaling and predispose to necrotizing fasciitis [ Cell Rep Med, 2024, 5(4):101503] PubMed: 38593810
OTULIN confers cisplatin resistance in osteosarcoma by mediating GPX4 protein homeostasis to evade the mitochondrial apoptotic pathway [ J Exp Clin Cancer Res, 2024, 43(1):330] PubMed: 39721999
Retinoic acid alleviates rotavirus-induced intestinal damage by regulating redox homeostasis and autophagic flux in piglets [ Anim Nutr, 2024, 20:16:409-421.] PubMed: 38371474
Retinoic acid alleviates rotavirus-induced intestinal damage by regulating redox homeostasis and autophagic flux in piglets [ Anim Nutr, 2024, 16:409-421] PubMed: 38371474
SARS-CoV-2 Omicron subvariants progressively adapt to human cells with altered host cell entry [ mSphere, 2024, 9(9):e0033824] PubMed: 39191389
Unveiling the Role of TMPRSS2 in the Proteolytic Activation of Pandemic and Zoonotic Influenza Viruses and Coronaviruses in Human Airway Cells [ Viruses, 2024, 16(11)1798] PubMed: 39599912
Lotus Sprout Extract Induces Selective Melanosomal Autophagy and Reduces Pigmentation [ J Cosmet Dermatol, 2024, 10.1111/jocd.16587] PubMed: 39305105

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.