Z-LEHD-FMK TFA

Catalog No.S7313 Batch:S731305

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Technical Data

Formula

C34H44F4N6O12
Molecular Weight 804.74 CAS No. 524746-03-0
Solubility (25°C)* In vitro DMSO 100 mg/mL (124.26 mM)
Ethanol 100 mg/mL (124.26 mM)
Water 50 mg/mL (62.13 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival.
Targets
Caspase-9 [1]
In vitro

Z-LEHD-FMK is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival[2].
In vivo

Z-LEHD-FMK TFA (Caspase-9 Inhibitor) is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HCT116 and SW480 cells

  • Concentrations

    20 μM

  • Incubation Time

    2 h

  • Method

    HCT116 and SW480 cells (as indicated) were pretreated with the inhibitors Z-IETD-FMK (caspase 8 inhibitor), Z-LEHD-FMK (caspase 9 inhibitor), Z-VDAVD-FMK (caspase 2 inhibitor), and Z-AEVD-FMK (caspase 10 inhibitor; 20 μm each) for 2 h, after which TRAIL (50 ng/ml) was added. After 4 h (or 16 h for caspase 3 activation) of TRAIL exposure, extracts were collected and analyzed by Western blotting for the expression of the different proteins.
Animal Study:

[3]
  • Animal Models

    Male Wistar albino rats

  • Dosages

    0.8 μM/kg

  • Administration

    i.v.

Selleck's Z-LEHD-FMK TFA has been cited by 24 publications

ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.] PubMed: 37646198
AMIGO2 attenuates innate cisplatin sensitivity by suppression of GSDME-conferred pyroptosis in non-small cell lung cancer [ J Cell Mol Med, 2023, 27(16):2412-2423] PubMed: 37438979
AMIGO2 attenuates innate cisplatin sensitivity by suppression of GSDME-conferred pyroptosis in non-small cell lung cancer [ J Cell Mol Med, 2023, 27(16):2412-2423] PubMed: 37438979
Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME [ Elife, 2022, 11e73792] PubMed: 35972780
Autophagy promotes GSDME-mediated pyroptosis via intrinsic and extrinsic apoptotic pathways in cobalt chloride-induced hypoxia reoxygenation-acute kidney injury [ Ecotoxicol Environ Saf, 2022, 242:113881] PubMed: 35863214
Physapruin A Enhances DNA Damage and Inhibits DNA Repair to Suppress Oral Cancer Cell Proliferation [ Int J Mol Sci, 2022, 23(16)8839] PubMed: 36012104
RhFGF21 Protects Epidermal Cells against UVB-Induced Apoptosis through Activating AMPK-Mediated Autophagy [ Int J Mol Sci, 2022, 23(20)12466] PubMed: 36293323
HCA -2-Hydroxy-Docosahexaenoic Acid Induces Apoptosis and Endoplasmic Reticulum Stress in Pancreatic Cancer Cells [ Int J Mol Sci, 2022, 23-179902] PubMed: 36077299
Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line [ Res Vet Sci, 2022, 143:124-133] PubMed: 35026629
Identification of ferroptosis as a novel mechanism for antitumor activity of natural product derivative a2 in gastric cancer [ Acta Pharm Sin B, 2021, 11(6):1513-1525] PubMed: 34221865

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.