YH239-EE

Catalog No.S7489 Batch:S748901

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Technical Data

Formula

C25H27Cl2N3O4
Molecular Weight 504.41 CAS No. 1364488-67-4
Solubility (25°C)* In vitro DMSO 100 mg/mL (198.25 mM)
Ethanol 18 mg/mL (35.68 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description YH239-EE, the ethyl ester of YH239, is a potent p53-MDM2 antagonist and an apoptosis inducer.
Targets
p53-MDM2 interaction [1]
In vitro YH239-EE inhibits the growth of OCI-AML-3 cells with wild type p53 by inhibiting the p53-MDM2 interaction. YH239-EE induces cell cycle arrest and causes potent cell apoptosis via activation of p53 and downstream targets in four AML cells (OCI-AML-3 and MOLM-13 with wt p53, NB4 with p53 mutation, and HL60 with p53 deletion). [1]

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    OCI-AML-3 cells

  • Concentrations

    ~50 μM

  • Incubation Time

    72 hours

  • Method

    Cell lines are harvested in log-phase growth and seeded following exposure to p53-MDM2 antagonists. Cell number and viability are determined by staining cells with Trypan blue 0.4% and counted with Countess slides.

Selleck's YH239-EE has been cited by 1 publication

Impaired Notch Signaling Leads to a Decrease in p53 Activity and Mitotic Catastrophe in Aged Muscle Stem Cells. [ Cell Stem Cell, 2018, 23(4):544-556] PubMed: 30244867

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.