Xanthoxyline

Catalog No.S4781 Batch:S478101

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Technical Data

Formula

C10H12O4
Molecular Weight 196.20 CAS No. 90-24-4
Solubility (25°C)* In vitro DMSO 39 mg/mL (198.77 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Xanthoxylin (Brevifolin), isolated from Zanthoxylum piperitum (Japanese pepper tree) and Sapium sebiferum (Chinese tallowtree), is a cytotoxic and fungicidal compound with the characteristics of a typical phytoalexin.
In vitro Xanthoxylin (RCX) exhibits potent cytotoxicity in a panel of different cancer cells. It presents IC50 values of 1.6 and 26.0 μM for the HCT116 and ACP-03 cancer cell lines, respectively. Xanthoxylin causes apoptosis in a time- and concentration-dependent manner and induces mitochondrial depolarization in HepG2 cells. It induces DNA intercalation, inhibited DNA synthesis and triggered the caspase-mediated apoptosis pathway in HepG2 cells, as observed by cell shrinkage, internucleosomal DNA fragmentation, externalization of phosphatidylserine, loss of mitochondrial transmembrane potential and activation of caspase-3, -8 and -9[1]. Xanthoxylin increases melanin production, number of dendrites, tyrosinase, and microphthalmia-associated transcription factor (MITF) expression in cultured B16F10 cells[2].
In vivo Xanthoxylin presents a potent in vivo antitumor effect in C.B-17 SCID mice engrafted with HepG2 cells[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    HepG2 cells

  • Concentrations

    12 and 24 μM

  • Incubation Time

    12, 24, 48 or 72 h

  • Method

    The cell viability of HepG2 cells is determined by the trypan blue staining after 12, 24, 48 and 72 h of incubation.
Animal Study:[1]
  • Animal Models

    C.B-17 severe combined immunodeficient (SCID) mice engrafted with HepG2 cells

  • Dosages

    2.5 and 5 mg/kg

  • Administration

    i.p.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.