Vincamine

Catalog No.S3891 Batch:S389101

Technical Data

Formula	C₂₁H₂₆N₂O₃
Molecular Weight	354.44	CAS No.	1617-90-9
Solubility (25°C)*	In vitro	DMSO	3 mg/mL (8.46 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Vincamine (Angiopac, Devincan, Equipur, Minorin, Novicet, Oxybral, Perval, Sostenil, Tripervan), an indole alkaloid found in the leaves of V. minor and C. roseus, is a peripheral vasodilator that increases blood flow to the brain.
In vitro	Vincamine decreases amyloid-β 25-35 induced oxidative stress and reduces reactive oxygen species levels. It significantly reduces the rate of apoptosis in a dose-dependent manner. Vincamine could provide neuroprotection from Aβ25-35 induced PC12 apoptosis through the phosphatidylinositol-3 kinase/Akt signaling pathway^[1].
In vivo	Vincamine could cross the blood-brain barrier, and its antioxidant scavenging capacity to inactivate hydroxyl free radicals is actually ranked in part with Vitamin E^[1]. Vincamine decreases the mean arterial blood pressure (by 5-35%), the cardial output and the heart rate. After oral application of 20 mg/kg vincamine in rats, vincamine is rapidly absorbed and eliminated. The apparent elimination half life is 1.71 h. The bioavailability of vincamine after oral administration is 58% suggesting a significant firstpass metabolism. Vincamine is taken up in a high concentration by the different organs, the maximal organ-plasma concentration ratio is 21 for lungs, 14.6 for brain, 14.3 for the kidneys, 8.9 for the liver and 7.6 for the heart. There is no steady-state between plasma and heart, lungs and brain, the elimination from these organs is significantly more rapid than from plasma^[2]. Vincamine is mainly metabolized in the liver and excreted by the kidneys and it may also go through cerebral metabolism. In the pharmacokinetic profile of vincamine, the compound is about 64% bound to plasma proteins, and 6% bound to erythrocytes^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines PC12 cells Concentrations 20, 40, 80 μM Incubation Time 3 h Method PC12 cells cultured in 6-well plates (4 × 10⁴ cells/well) for 24 h are treated with various concentrations of vincamine for 3 h before the addition of 30 μM Aβ25-35 and further 24 h incubation. Cells are then digested with trypsin and washed twice in phosphate buffered solution (PBS). Thereafter, cells are suspended in 500 μl of PBS and lysed by ultrasonication in the presence of protease inhibitor before centrifugation at 4000 rpm for 5 min. The supernatant is collected for analysis.
Animal Study:^{^[3]}	Animal Models male Sprague-Dawley rats Dosages 10 and 30 mg/kg Administration i.v.

Cell Assay:^{^[1]}

Cell lines
PC12 cells
Concentrations
20, 40, 80 μM
Incubation Time
3 h
Method
PC12 cells cultured in 6-well plates (4 × 10⁴ cells/well) for 24 h are treated with various concentrations of vincamine for 3 h before the addition of 30 μM Aβ25-35 and further 24 h incubation. Cells are then digested with trypsin and washed twice in phosphate buffered solution (PBS). Thereafter, cells are suspended in 500 μl of PBS and lysed by ultrasonication in the presence of protease inhibitor before centrifugation at 4000 rpm for 5 min. The supernatant is collected for analysis.

Animal Study:^{^[3]}

Animal Models
male Sprague-Dawley rats
Dosages
10 and 30 mg/kg
Administration
i.v.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.