Trans-Zeatin

Catalog No.S4884 Batch:S488401

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Technical Data

Formula

C10H13N5O

Molecular Weight 219.24 CAS No. 1637-39-4
Solubility (25°C)* In vitro DMSO 43 mg/mL (196.13 mM)
Ethanol 2 mg/mL (9.12 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Trans-Zeatina ((E)-Zeatin) is the member of the plant growth hormone family known as cytokinins, which regulate cell division, development, and nutrient processing. Trans-Zeatin inhibits UVB-induced MMP-1 expression, c-Jun activation and phosphorylation of ERK, JNK and p38 MAP kinases (MAPKs) dose-dependently.
Targets
MMP-1 [1] ERK [1] JNK [1] p38 MAPK [1]
In vitro Pretreatment with trans-Zeatin significantly inhibits UVB-induced MMP-1 expression and c-Jun activation in a dose-dependent manner in cultured human skin fibroblasts, which may be mediated by inhibition of ERK, JNK and p38 MAPKs signaling pathways[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    human skin fibroblasts (HSFs)

  • Concentrations

    20, 40, 80, 150, 300, 600 μM

  • Incubation Time

    24 h

  • Method

    HSFs are seeded in a 96-well plate. After various concentrations of trans-Zeatin or UVB treatment, cells are cultured in 100 μl 10% DMEM in each well for the indicated time. MTT solution (10 μl) at a concentration of 5 mg/ml is added to each well, and the cells are then incubated at 37˚C for 4 h. The medium is removed, and 200 μl of DMSO is added into each well to dissolve formazan crystals by pipetting up and down several times. The absorbance is measured spectrophotometrically on an ELISA plate reader at a wavelength of 570 nm.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.