Thymoquinone

Catalog No.S4761 Batch:S476102

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Technical Data

Formula

C10H12O2
Molecular Weight 164.20 CAS No. 490-91-5
Solubility (25°C)* In vitro DMSO 33 mg/mL (200.97 mM)
Ethanol 33 mg/mL (200.97 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
1.65mg/ml Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.65mg/ml Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Thymoquinone (TQ) is the bioactive phytochemical constituent of the seeds oil of Nigella sativa with anti-inflammatory and anti-oxidant effects.
In vitro Thymoquinone (TQ) strongly inhibits the production of nitric oxide (NO) and repressed NO synthase (iNOS), tumor necrosis factor (TNF)-α, cyclooxygenase (COX)−2, interleukin (IL)−6, and IL-1β expression in LPS-activated RAW264.7 cells. TQ acts as a radical scavenger and thereby preserves the enzymatic activity of antioxidants like catalase, glutathione peroxidase, and glutathione-S-transferase. TQ abolishes the activity of IRAK1, but not IRAK4, thereby reducing the activity of downstream NF-κB and AP-1[1]. Treatment with TQ significantly decreases cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment significantly decreases the level of phosphorylated p65 in the nucleus, which indicates the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreases the expression levels of VEGF, c-Myc and Bcl-2[2].
In vivo Treatment of LPS/D-GalN–induced hepatitis and EtOH/HCl–induced gastritis mouse models with TQ significantly ameliorates disease symptoms[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    RAW264.7 cells, peritoneal macrophages, and HEK293 cells

  • Concentrations

    6.25, 12.5, 25 μM

  • Incubation Time

    24 h

  • Method

    The cell viability of RAW264.7 cells, peritoneal macrophages, and HEK293 cells during TQ exposure for 24 h is determined by a conventional MTT assay.
Animal Study:

[1]
  • Animal Models

    an HCl/EtOH-induced gastritis mouse model (Male C57BL/6 and ICR mice)

  • Dosages

    5 and 25 mg/kg

  • Administration

    Oral administration

Selleck's Thymoquinone has been cited by 3 publications

PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal Cancer [ Int J Mol Sci, 2022, 23(4)2305] PubMed: 35216429
Potential food-drug interaction risk of thymoquinone with warfarin [ Chem Biol Interact, 2022, 365:110070] PubMed: 35921950
Potential herb-drug interaction risk of thymoquinone and phenytoin [ Chem Biol Interact, 2022, 353:109801] PubMed: 34998822

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.