Tetrahydropalmatine hydrochloride

Catalog No.S3854 Batch:S385401

Technical Data

Formula	C₂₁H₂₅NO₄.HCl
Molecular Weight	391.89	CAS No.	6024-85-7
Solubility (25°C)*	In vitro	DMSO	12 mg/mL (30.62 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Tetrahydropalmatine (THP, Gindarine, 1-Tetrahydropalmitine) is an isoquinoline alkaloid found in several different plant species, mainly in the Corydalis genus (Yan Hu Suo), but also in other plants such as Stephania rotunda. It is a potent muscle relaxant. Tetrahydropalmatine hydrochloride acts through inhibition of amygdaloid release of dopamine to inhibit an epileptic attack in rats.
In vitro	Tetrahydropalmatine inhibits the LPSinduced production of COX-2 protein and mRNA in a dose-dependent manner. It suppresses activation of the NF-kB signaling pathway, which results in regulation of IL-8 expression and specifically blocks the ERK1=2 and p38 MAPK signaling pathways^[1]. L-THP results in p53 independent apoptosis via down-regulating XIAP protein by inhibiting MDM2 associated with proteasome-dependent pathway and increased sensitivity of EU-4 cells against doxorubicin. L-THP causes activation of caspase and resulted in apoptosis^[2]. l-THP possesses a blocking effect on dopamine D1 and D2 receptors and voltage-sensitive Ca2+ channels^[3].
In vivo	l-THP could attenuate the locomotor-stimulating effects of oxycodone and inhibit the development and expression of oxycodone behavioral sensitization^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines THP-1 cells Concentrations 0.2, 1, or 2 mM Incubation Time 24 h Method Cell viability is examined by MTT assay. In brief, THP-1 cells are seeded at a density of 5×10⁴ cells/mL in 96-well plates. Each batch of cells includes a nontreated group as a control. Tetrahydropalmatine (0.2, 1, or 2 mM) and LPS (2 mg/mL) are then added to each well, after which the plates are incubated for 24 hours at 37℃ under 5% CO2. Next, MTT solutions (5 mg/mL) are added to each well, and the samples are then cultured for an additional 4 hours. The supernatant is then discarded, and 100 mL of dimethylsulfoxide is added to each well. Next, the optical density is read at 590 nm.
Animal Study:^{^[3]}	Animal Models Kunming mice Dosages 6.25, 12.5, and 18.75 mg/kg Administration administered intragastrically

Cell Assay:^{^[1]}

Cell lines
THP-1 cells
Concentrations
0.2, 1, or 2 mM
Incubation Time
24 h
Method
Cell viability is examined by MTT assay. In brief, THP-1 cells are seeded at a density of 5×10⁴ cells/mL in 96-well plates. Each batch of cells includes a nontreated group as a control. Tetrahydropalmatine (0.2, 1, or 2 mM) and LPS (2 mg/mL) are then added to each well, after which the plates are incubated for 24 hours at 37℃ under 5% CO2. Next, MTT solutions (5 mg/mL) are added to each well, and the samples are then cultured for an additional 4 hours. The supernatant is then discarded, and 100 mL of dimethylsulfoxide is added to each well. Next, the optical density is read at 590 nm.

Animal Study:^{^[3]}

Animal Models
Kunming mice
Dosages
6.25, 12.5, and 18.75 mg/kg
Administration
administered intragastrically

References

Selleck's Tetrahydropalmatine hydrochloride has been cited by 1 publication

Tetrahydropalmatine exerts analgesic effects by promoting apoptosis and inhibiting the activation of glial cells in rats with inflammatory pain [ Mol Pain, 2021, 17:17448069211042117]	PubMed: 34505815

Tetrahydropalmatine exerts analgesic effects by promoting apoptosis and inhibiting the activation of glial cells in rats with inflammatory pain [ Mol Pain, 2021, 17:17448069211042117]

PubMed: 34505815

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SHIPPING AND STORAGE
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